ArticleWorld journal of microbiology & biotechnology2014
Development of a SCAR marker for molecular detection and diagnosis of Tilletia controversa Kühn, the causal fungus of wheat dwarf bunt.
Article in World journal of microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Characterization of a Small Cysteine-Rich Secreted Effector, TcSCP_9014, inPlants (Basel, Switzerland) · 2024Article
- Development of SCAR Markers for Genetic Authentication ofJournal of fungi (Basel, Switzerland) · 2024Article
- Kernel Transcriptome Profiles of Susceptible Wheat Genotypes in Response to Wheat Dwarf Bunt.International journal of molecular sciences · 2023Article
- Intelligent reprogramming of wheat for enhancement of fungal and nematode disease resistance using advanced molecular techniques.Frontiers in plant science · 2023Review
- ITRAQ-Based Proteomic Analysis of Wheat (Biology · 2022Article
- Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores.Applied microbiology and biotechnology · 2022Article
- Characterization of Rhizosphere Microbial Communities for Disease Incidence and Optimized Concentration of Difenoconazole Fungicide for Controlling of Wheat Dwarf Bunt.Frontiers in microbiology · 2022Article
- Development of a loop-mediated isothermal amplification assay for the detection of Tilletia controversa based on genome comparison.Scientific reports · 2021Article
- Wheat Varietal Response toGenes · 2021Article
- Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat.Scientific reports · 2021Article
- Characterization of histological changes at the tillering stage (Z21) in resistant and susceptible wheat plants infected by Tilletia controversa Kühn.BMC plant biology · 2021Article
- Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt.Scientific reports · 2020Article
- Methyljasmonate and salicylic acid contribute to the control of Tilletia controversa Kühn, causal agent of wheat dwarf bunt.Scientific reports · 2020Article
- Development of droplet digital PCR for the detection of Tilletia laevis, which causes common bunt of wheat, based on the SCAR marker derived from ISSR and real-time PCR.Scientific reports · 2020Article
- Development of the Droplet Digital PCR to Detect the Teliospores ofFrontiers in microbiology · 2020Article
- Development of ISSR-derived SCAR Marker and SYBR Green I Real-time PCR Method for Detection of Teliospores of Tilletia laevis Kühn.Scientific reports · 2019Article
- Genome sequencing and comparison of fiveIMA fungus · 2019Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tilletia controversa Kühn (TCK) is an important quarantine pathogen that causes wheat dwarf bunt and results in devastating damage to wheat production. The fungus is difficult to be distinguished from T. caries and T. laevis, which cause wheat common bunt, based on morphological, physiological and symptomatological characteristics of the pathogens. The traditional detection of the fungus can be a long and tedious process with poor accuracy. The inter-simple sequence repeat (ISSR) technique has been used for identifying molecular markers for detection of TCK. Of 28 ISSR primers screened, ISSR-859 amplified a specific 678 bp DNA fragment from all TCK isolates but not from any isolates of the common bunt fungi or other pathogenic fungi tested. Based on the fragment sequence, a pair of sequence characterized amplified region (SCAR) primers was designed, which amplified a 372 bp DNA fragment specifically in TCK. The SCAR marker was detected using as low as 1 ng template DNA of TCK, and was also detected using broken teliospores and DNA from asymptomatic wheat samples. We developed the SYBR Green I and TaqMan Green I and TaqMan real-time polymorphism chain reaction methods to detect TCK with the detection limit of 0.1 fg with asymptomatic wheat samples. Further work is needed to develop a rapid test kit for this pathogenic fungus using the designed specific primers.
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