ArticleScientific reports2014
Optical trapping reveals propulsion forces, power generation and motility efficiency of the unicellular parasites Trypanosoma brucei brucei.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- How much (ATP) does it cost to build a trypanosome? A theoretical study on the quantity of ATP needed to maintain and duplicate a bloodstream-form Trypanosoma brucei cell.PLoS pathogens · 2023Article
- Spatial confinement of Trypanosoma brucei in microfluidic traps provides a new tool to study free swimming parasites.PloS one · 2023Article
- Light chain 2 is a Tctex-type related axonemal dynein light chain that regulates directional ciliary motility in Trypanosoma brucei.PLoS pathogens · 2022Article
- Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation.Scientific reports · 2021Article
- Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics.Micromachines · 2020Review
- Dynamic force measurements on swimmingJournal of the Royal Society, Interface · 2020Article
- Frontiers in Microfluidics, a Teaching Resource Review.Bioengineering (Basel, Switzerland) · 2019Review
- A Microfluidic-Based Microscopy Platform for Continuous Interrogation of Trypanosoma brucei during Environmental Perturbation.Biochemistry · 2019Article
- The Fantastic Voyage of the Trypanosome: A Protean Micromachine Perfected during 500 Million Years of Engineering.Micromachines · 2018Review
- Microfluidics-Based Approaches to the Isolation of African Trypanosomes.Pathogens (Basel, Switzerland) · 2017Article
- Article
- Extending calibration-free force measurements to optically-trapped rod-shaped samples.Scientific reports · 2017Article
- Mode instabilities and dynamic patterns in a colony of self-propelled surfactant particles covering a thin liquid layer.The European physical journal. E, Soft matter · 2016Article
- Knockdown of Inner Arm Protein IC138 in Trypanosoma brucei Causes Defective Motility and Flagellar Detachment.PloS one · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unicellular parasites have developed sophisticated swimming mechanisms to survive in a wide range of environments. Cell motility of African trypanosomes, parasites responsible for fatal illness in humans and animals, is crucial both in the insect vector and the mammalian host. Using millisecond-scale imaging in a microfluidics platform along with a custom made optical trap, we are able to confine single cells to study trypanosome motility. From the trapping characteristics of the cells, we determine the propulsion force generated by cells with a single flagellum as well as of dividing trypanosomes with two fully developed flagella. Estimates of the dissipative energy and the power generation of single cells obtained from the motility patterns of the trypanosomes within the optical trap indicate that specific motility characteristics, in addition to locomotion, may be required for antibody clearance. Introducing a steerable second optical trap we could further measure the force, which is generated at the flagellar tip. Differences in the cellular structure of the trypanosomes are correlated with the trapping and motility characteristics and in consequence with their propulsion force, dissipative energy and power generation.
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