ArticlePloS one2014
Signaling of pigment-dispersing factor (PDF) in the Madeira cockroach Rhyparobia maderae.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Neurons sensitive to sky compass signals in the brain of the Madeira cockroach Rhyparobia maderae.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Article
- Clock-related neuropeptides inFrontiers in genetics · 2026Article
- Review
- Mutual coupling of neurons in the circadian master clock: What we can learn from fruit flies.Neurobiology of sleep and circadian rhythms · 2025Article
- The Never Given 2022 Pittendrigh/Aschoff Lecture: The Clock Network in the Brain-Insights From Insects.Journal of biological rhythms · 2025Article
- Pigment-dispersing factor is present in circadian clock neurons of pea aphids and may mediate photoperiodic signalling to insulin-producing cells.Open biology · 2023Article
- Contribution of membrane-associated oscillators to biological timing at different timescales.Frontiers in physiology · 2023Article
- Analysis of Pigment-Dispersing Factor Neuropeptides and Their Receptor in a Velvet Worm.Frontiers in endocrinology · 2020Article
- Molecular and circuit mechanisms mediating circadian clock output in the Drosophila brain.The European journal of neuroscience · 2020Review
- Flies as models for circadian clock adaptation to environmental challenges.The European journal of neuroscience · 2020Review
- Model and Non-model Insects in Chronobiology.Frontiers in behavioral neuroscience · 2020Review
- Beyond spikes: Multiscale computational analysis ofNetwork neuroscience (Cambridge, Mass.) · 2019Article
- The CCHamide1 Neuropeptide Expressed in the Anterior Dorsal Neuron 1 Conveys a Circadian Signal to the Ventral Lateral Neurons inFrontiers in physiology · 2018Article
- Pigment-Dispersing Factor-expressing neurons convey circadian information in the honey bee brain.Open biology · 2018Article
- A Series of Suppressive Signals within the Drosophila Circadian Neural Circuit Generates Sequential Daily Outputs.Neuron · 2017Article
- Neuronal circadian clock protein oscillations are similar in behaviourally rhythmic forager honeybees and in arrhythmic nurses.Open biology · 2017Article
- Fluorescence circadian imaging reveals a PDF-dependent transcriptional regulation of the Drosophila molecular clock.Scientific reports · 2017Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The insect neuropeptide pigment-dispersing factor (PDF) is a functional ortholog of vasoactive intestinal polypeptide, the coupling factor of the mammalian circadian pacemaker. Despite of PDF's importance for synchronized circadian locomotor activity rhythms its signaling is not well understood. We studied PDF signaling in primary cell cultures of the accessory medulla, the circadian pacemaker of the Madeira cockroach. In Ca²⁺ imaging studies four types of PDF-responses were distinguished. In regularly bursting type 1 pacemakers PDF application resulted in dose-dependent long-lasting increases in Ca²⁺ baseline concentration and frequency of oscillating Ca²⁺ transients. Adenylyl cyclase antagonists prevented PDF-responses in type 1 cells, indicating that PDF signaled via elevation of intracellular cAMP levels. In contrast, in type 2 pacemakers PDF transiently raised intracellular Ca²⁺ levels even after blocking adenylyl cyclase activity. In patch clamp experiments the previously characterized types 1-4 could not be identified. Instead, PDF-responses were categorized according to ion channels affected. Application of PDF inhibited outward potassium or inward sodium currents, sometimes in the same neuron. In a comparison of Ca²⁺ imaging and patch clamp experiments we hypothesized that in type 1 cells PDF-dependent rises in cAMP concentrations block primarily outward K⁺ currents. Possibly, this PDF-dependent depolarization underlies PDF-dependent phase advances of pacemakers. Finally, we propose that PDF-dependent concomitant modulation of K⁺ and Na⁺ channels in coupled pacemakers causes ultradian membrane potential oscillations as prerequisite to efficient synchronization via resonance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.