ArticlePloS one2014
Characterizing the roles of Cryphonectria parasitica RNA-dependent RNA polymerase-like genes in antiviral defense, viral recombination and transposon transcript accumulation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
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- A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference.PLoS pathogens · 2023Article
- Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities.Functional & integrative genomics · 2023Review
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- PepYLCIV and PepYLCAV resistance gene Pepy-2 encodes DFDGD-Class RNA-dependent RNA polymerase in Capsicum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Essential role of γ-clade RNA-dependent RNA polymerases in rice development and yield-related traits is linked to their atypical polymerase activities regulating specific genomic regions.The New phytologist · 2021Article
- Identification of an RNA Silencing Suppressor Encoded by a Symptomless Fungal Hypovirus, Cryphonectria Hypovirus 4.Biology · 2021Article
- Establishment of Neurospora crassa as a model organism for fungal virology.Nature communications · 2020Article
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- Early Transcriptional Response to DNA Virus Infection inViruses · 2019Article
- Dicer functions transcriptionally and posttranscriptionally in a multilayer antiviral defense.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Comprehensive analysis of full genome sequence andIMA fungus · 2019Article
- Roles of Argonautes and Dicers onFrontiers in plant science · 2019Article
- Differential Contribution of RNA Interference Components in Response to Distinct Fusarium graminearum Virus Infections.Journal of virology · 2018Article
- Deep sequencing of mycovirus-derived small RNAs from Botrytis species.Molecular plant pathology · 2017Article
- Differential Inductions of RNA Silencing among Encapsidated Double-Stranded RNA Mycoviruses in the White Root Rot Fungus Rosellinia necatrix.Journal of virology · 2016Article
- Small RNA-Based Antiviral Defense in the Phytopathogenic Fungus Colletotrichum higginsianum.PLoS pathogens · 2016Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
An inducible RNA-silencing pathway, involving a single Dicer protein, DCL2, and a single Argonaute protein, AGL2, was recently shown to serve as an effective antiviral defense response in the chestnut blight fungus Cryphonectria parasitica. Eukaryotic RNA-dependent RNA polymerases (RdRPs) are frequently involved in transcriptional and posttranscriptional gene silencing and antiviral defense. We report here the identification and characterization of four RdRP genes (rdr1-4) in the C. parasitica genome. Sequence relationships with other eukaryotic RdRPs indicated that RDR1 and RDR2 were closely related to QDE-1, an RdRP involved in RNA silencing ("quelling") in Neurospora crassa, whereas RDR3 was more closely related to the meiotic silencing gene SAD-1 in N. crassa. The RdRP domain of RDR4, related to N. crassa RRP-3 of unknown function, was truncated and showed evidence of alternative splicing. Similar to reports for dcl2 and agl2, the expression levels for rdr3 and rdr4 increased after hypovirus CHV-1/EP713 infection, while expression levels of rdr1 and rdr2 were unchanged. The virus-responsive induction patterns for rdr3 and rdr4 were altered in the Δdcl2 and Δagl2 strains, suggesting some level of interaction between rdr3 and rdr4 and the dcl2/agl2 silencing pathway. Single rdr gene knockouts Δrdr1-4, double knockouts Δrdr1/2, Δrdr2/3, Δrdr1/3, and a triple knockout, Δrdr1/2/3, were generated and evaluated for effects on fungal phenotype, the antiviral defense response, viral RNA recombination activity and transposon expression. None of the single or multiple rdr knockout strains displayed any phenotypic differences from the parental strains with or without viral infection or any significant changes in viral RNA accumulation or recombination activity or transposon RNA accumulation, indicating no detectable contribution by the C. parasitica rdr genes to these processes.
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