ArticlePLoS biology2014
Differentially timed extracellular signals synchronize pacemaker neuron clocks.
Article in PLoS biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface.Npj imaging · 2026Article
- Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface.bioRxiv : the preprint server for biology · 2026Article
- A functional clock in only two dorsal clock neurons is sufficient to restore the basal circadian activity pattern ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mutual coupling of neurons in the circadian master clock: What we can learn from fruit flies.Neurobiology of sleep and circadian rhythms · 2025Article
- Tango-seq: overlaying transcriptomics on connectomics to identify neurons downstream ofbioRxiv : the preprint server for biology · 2024Article
- Circadian Rhythm Regulation by Pacemaker Neuron Chloride Oscillation in Flies.Physiology (Bethesda, Md.) · 2024Review
- Glutamate and clock help bean bugs track seasonal reproductive changes.PLoS biology · 2022Article
- Chloride oscillation in pacemaker neurons regulates circadian rhythms through a chloride-sensing WNK kinase signaling cascade.Current biology : CB · 2022Article
- Dorsal clock neurons iniScience · 2021Article
- Sleep drive reconfigures wake-promoting clock circuitry to regulate adaptive behavior.PLoS biology · 2021Article
- Sites of Circadian Clock Neuron Plasticity Mediate Sensory Integration and Entrainment.Current biology : CB · 2020Article
- Entrainment of theNeuroscience insights · 2020Review
- Article
- Neuronal Activity in Non-LNv Clock Cells Is Required to Produce Free-Running Rest:Activity Rhythms in Drosophila.Journal of biological rhythms · 2019Article
- A Symphony of Signals: Intercellular and Intracellular Signaling Mechanisms Underlying Circadian Timekeeping in Mice and Flies.International journal of molecular sciences · 2019Review
- Article
- Reconfiguration of a Multi-oscillator Network by Light in the Drosophila Circadian Clock.Current biology : CB · 2018Article
- The CCHamide1 Neuropeptide Expressed in the Anterior Dorsal Neuron 1 Conveys a Circadian Signal to the Ventral Lateral Neurons inFrontiers in physiology · 2018Article
- Article
- SIK3-HDAC4 signaling regulatesProceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
10 authors.
Funding
Abstract
Synchronized neuronal activity is vital for complex processes like behavior. Circadian pacemaker neurons offer an unusual opportunity to study synchrony as their molecular clocks oscillate in phase over an extended timeframe (24 h). To identify where, when, and how synchronizing signals are perceived, we first studied the minimal clock neural circuit in Drosophila larvae, manipulating either the four master pacemaker neurons (LNvs) or two dorsal clock neurons (DN1s). Unexpectedly, we found that the PDF Receptor (PdfR) is required in both LNvs and DN1s to maintain synchronized LNv clocks. We also found that glutamate is a second synchronizing signal that is released from DN1s and perceived in LNvs via the metabotropic glutamate receptor (mGluRA). Because simultaneously reducing Pdfr and mGluRA expression in LNvs severely dampened Timeless clock protein oscillations, we conclude that the master pacemaker LNvs require extracellular signals to function normally. These two synchronizing signals are released at opposite times of day and drive cAMP oscillations in LNvs. Finally we found that PdfR and mGluRA also help synchronize Timeless oscillations in adult s-LNvs. We propose that differentially timed signals that drive cAMP oscillations and synchronize pacemaker neurons in circadian neural circuits will be conserved across species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.