ArticlePloS one2014
Involvement of the leaf-specific multidrug and toxic compound extrusion (MATE) transporter Nt-JAT2 in vacuolar sequestration of nicotine in Nicotiana tabacum.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 81 citations in OpenAlex.
- Characterization of Nucleobase Cation Symporter 1 (NCS1) Localized to the Plastids of Nicotiana tabacum.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- Cell-type-specific alkaloid and terpenoid biosynthesis in glandular trichomes: single-cell and spatial transcriptomic perspectives.Frontiers in plant science · 2026Review
- Overexpression of NtGSTU10 gene enhances nicotine content in hybrids by promoting the synthesis and transport metabolism of nicotine.BMC biotechnology · 2025Article
- Transport of secondary metabolites in plants: Mechanistic insights and transporter engineering for crop improvement.Plant communications · 2025Review
- A Multidrug and Toxic Compound Extrusion Transporter, RgMATE6, Facilitates Vacuolar Transport of Acteoside inPlants (Basel, Switzerland) · 2025Article
- Transportation engineering for enhanced production of plant natural products in microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Multidrug and Toxic Compound Extrusion Transporters: Ubiquitous Multifaceted Proteins in Microbes, Plants, and Their Interactions.Microorganisms · 2024Review
- The formation of nicotine heterosis is mainly achieved by enhancing the nicotine transport capacity in hybrids.BMC plant biology · 2024Article
- Integration of co-culture and transport engineering for enhanced metabolite production.Plant biotechnology (Tokyo, Japan) · 2024Article
- Characterization of a vacuolar importer of secologanin in Catharanthus roseus.Communications biology · 2024Article
- Identification ofInternational journal of molecular sciences · 2024Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Radiocarbon Flux Measurements Reveal Mechanistic Insight into Heat-Stress Induction of Nicotine Biosynthesis inInternational journal of molecular sciences · 2023Article
- Terpenoid Transport in Plants: How Far from the Final Picture?Plants (Basel, Switzerland) · 2023Review
- The Identification ofGenes · 2022Article
- Genome-Wide Analysis of Multidrug and Toxic Compound Extruction Transporters in Grape.Frontiers in plant science · 2022Article
- A Systematic Phylogenomic Classification of the Multidrug and Toxic Compound Extrusion Transporter Gene Family in Plants.Frontiers in plant science · 2022Article
- Transport engineering for improving the production and secretion of valuable alkaloids inMetabolic engineering communications · 2021Article
- An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport inInternational journal of molecular sciences · 2021Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkaloids play a key role in higher plant defense against pathogens and herbivores. Following its biosynthesis in root tissues, nicotine, the major alkaloid of Nicotiana species, is translocated via xylem transport toward the accumulation sites, leaf vacuoles. Our transcriptome analysis of methyl jasmonate-treated tobacco BY-2 cells identified several multidrug and toxic compound extrusion (MATE) transporter genes. In this study, we characterized a MATE gene, Nicotiana tabacum jasmonate-inducible alkaloid transporter 2 (Nt-JAT2), which encodes a protein that has 32% amino acid identity with Nt-JAT1. Nt-JAT2 mRNA is expressed at a very low steady state level in whole plants, but is rapidly upregulated by methyl jasmonate treatment in a leaf-specific manner. To characterize the function of Nt-JAT2, yeast cells were used as the host organism in a cellular transport assay. Nt-JAT2 was localized at the plasma membrane in yeast cells. When incubated in nicotine-containing medium, the nicotine content in Nt-JAT2-expressing cells was significantly lower than in control yeast. Nt-JAT2-expressing cells also showed lower content of other alkaloids like anabasine and anatabine, but not of flavonoids, suggesting that Nt-JAT2 transports various alkaloids including nicotine. Fluorescence assays in BY-2 cells showed that Nt-JAT2-GFP was localized to the tonoplast. These findings indicate that Nt-JAT2 is involved in nicotine sequestration in leaf vacuoles following the translocation of nicotine from root tissues.
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