ArticleAnnals of biomedical engineering2014
Amniotic fluid-derived stem cells demonstrated cardiogenic potential in indirect co-culture with human cardiac cells.
Article in Annals of biomedical engineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System.Biosensors · 2021Article
- Controlled Release of Small Molecules for Cardiac Differentiation of Pluripotent Stem Cells.Tissue engineering. Part A · 2018Article
- Cardiomyocytes Derived from HumanScientific reports · 2018Article
- Biological characterization and pluripotent identification of ovine amniotic fluid stem cells.Cytotechnology · 2018Article
- Protective effect of GDNF-engineered amniotic fluid-derived stem cells on the renal ischaemia reperfusion injury in vitro.Cell proliferation · 2018Article
- Cardiac Restoration Stemming From the Placenta Tree: Insights From Fetal and Perinatal Cell Biology.Frontiers in physiology · 2018Review
- Tat-MyoD fused proteins, together with C2c12 conditioned medium, are able to induce equine adult mesenchimal stem cells towards the myogenic fate.Veterinary research communications · 2017Article
- Differentiation of spontaneously contracting cardiomyocytes from non-virally reprogrammed human amniotic fluid stem cells.PloS one · 2017Article
- Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases.International journal of molecular sciences · 2016Review
- Muscle Decline in Aging and Neuromuscular Disorders - Mechanisms and Countermeasures: Terme Euganee, Padova (Italy), April 13-16, 2016.European journal of translational myology · 2016Article
- Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression.International journal of nanomedicine · 2016Article
- Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases.Brain circulationReview
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Amniotic fluid-derived stem cells (AFSC) have been shown to be broadly multipotent and non-tumorogenic. Previous studies of direct mixing of AFSC and neonatal rat ventricle myocytes indicated evidence of AFSC cardiogenesis. In this study, we examined human AFSC cardiogenic potential in indirect co-culture with human cardiac cells in conditions that eliminated the possibility of cell fusion. Human AFSC in contact with human cardiac cells showed expression of cardiac troponin T (cTnT) in immunohistochemistry, and no evidence of cell fusion was found through fluorescent in situ hybridization. When indirectly co-cultured with cardiac cells, human AFSC in contact with cardiac cells across a thin porous membrane showed a statistically significant increase in cTnT expression compared to non-contact conditions but lacked upregulation of calcium modulating proteins and did not have functional or morphological characteristics of mature cardiomyocytes. This suggests that contact is a necessary but not sufficient condition for AFSC cardiac differentiation in co-culture with cardiac cells.
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