ArticleMolecular reproduction and development2014
Depletion of pericentrin in mouse oocytes disrupts microtubule organizing center function and meiotic spindle organization.
Article in Molecular reproduction and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Identification of 19 new risk loci and potential regulatory mechanisms influencing susceptibility to testicular germ cell tumor.Nature genetics · 2017Pooled it
- A human CEP120 gene variant impairs meiotic spindle building causing aneuploidy†.Biology of reproduction · 2026Article
- Cumulus Expansion and Oocyte Quality Across the Pubertal Transition in Mice: Implications for Modeling Fertility Preservation in Adolescents.Research square · 2026Article
- A MOPD II-associated Pericentrin variant disrupts PACT domain dimerization and pericentriolar material recruitment.bioRxiv : the preprint server for biology · 2026Article
- Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation.Nature communications · 2025Article
- Spatio-temporal requirements of Aurora kinase A in mouse oocyte meiotic spindle building.iScience · 2024Article
- The cGAS-STING/PERK-eIF2α: Individual or Potentially Collaborative Signaling Transduction in Cardiovascular Diseases.International journal of biological sciences · 2024Review
- Pericentrin deficiency in smooth muscle cells augments atherosclerosis through HSF1-driven cholesterol biosynthesis and PERK activation.JCI insight · 2023Article
- Pericentric major satellite transcription is essential for meiotic chromosome stability and spindle pole organization.Open biology · 2023Article
- PAK1 Is Involved in the Spindle Assembly during the First Meiotic Division in Porcine Oocytes.International journal of molecular sciences · 2023Article
- Chromosome Segregation in the Oocyte: What Goes Wrong during Aging.International journal of molecular sciences · 2022Review
- Acentriolar spindle assembly in mammalian female meiosis and the consequences of its perturbations on human reproduction†.Biology of reproduction · 2022Review
- Microtubule organizing centers regulate spindle positioning in mouse oocytes.Developmental cell · 2022Article
- Loss of acentriolar MTOCs disrupts spindle pole Aurora A and assembly of the liquid-like meiotic spindle domain in oocytes.Journal of cell science · 2021Article
- Aurora kinase A is essential for meiosis in mouse oocytes.PLoS genetics · 2021Article
- Two mechanisms drive pronuclear migration in mouse zygotes.Nature communications · 2021Article
- Hexestrol Deteriorates Oocyte Quality via Perturbation of Mitochondrial Dynamics and Function.Frontiers in cell and developmental biology · 2021Article
- Electrofusion Stimulation Is an Independent Factor of Chromosome Abnormality in Mice Oocytes ReconstructedFrontiers in endocrinology · 2021Article
- Echinoderm Microtubule Associated Protein Like 1 Is Indispensable for Oocyte Spindle Assembly and Meiotic Progression in Mice.Frontiers in cell and developmental biology · 2021Article
- Exome sequencing links CEP120 mutation to maternally derived aneuploid conception risk.Human reproduction (Oxford, England) · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Accurate chromosome segregation is dependent on the formation and stability of the microtubule spindle apparatus. Meiotic spindle assembly in oocytes differs from the process used during mitosis, and is regulated by unique microtubule organizing centers (MTOCs) that lack centrioles. To gain insight into the molecular composition and function of acentriolar MTOCs in mouse oocytes, we assessed the role of a key MTOC-associated protein, pericentrin (PCNT). In somatic cells, pericentrin functions as a scaffold that binds specific proteins at MTOCs, including γ-tubulin, which is necessary for microtubule nucleation. Pericentrin is expressed in oocytes, but the conservation of its function is not known. Pericentrin localizes specifically to MTOCs during prophase-I arrest in mouse oocytes recovered from pre-ovulatory ovarian follicles, and remains associated with MTOCs at spindle poles during metaphase-I and -II. To test function, specific siRNAs were used to knock down Pcnt transcripts in mouse oocytes. Efficient protein depletion was confirmed by Western blot as well as immunofluorescence analysis. Notably, meiotic spindle structure and chromosome alignment were disrupted in Pcnt-depleted oocytes. Disorganized spindle structures with reduced microtubule density and misaligned chromosomes were observed in the majority of these oocytes (∼ 70%). In addition, γ-tubulin localization to MTOCs was significantly reduced and microtubule regrowth, following cold treatment, was delayed in Pcnt-depleted oocytes. Thus, pericentrin is a key functional component of the unique acentriolar MTOCs of mouse oocytes, and plays an important role in regulating meiotic spindle assembly and/or stability.
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