ArticleMolecular & cellular proteomics : MCP2014
Quantitative phosphoproteomics of cytotoxic T cells to reveal protein kinase d 2 regulated networks.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- A Rapid Translational Immune Response Program in CD8 Memory T Lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- LymphoAtlas: a dynamic and integrated phosphoproteomic resource of TCR signaling in primary T cells reveals ITSN2 as a regulator of effector functions.Molecular systems biology · 2020Article
- IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics.PLoS biology · 2020Article
- Phospholipids: Pulling Back the Actin Curtain for Granule Delivery to the Immune Synapse.Frontiers in immunology · 2019Review
- Lysophosphatidic acid via LPA-receptor 5/protein kinase D-dependent pathways induces a motile and pro-inflammatory microglial phenotype.Journal of neuroinflammation · 2017Article
- Phosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells.Frontiers in immunology · 2017Article
- Lighting Up T Lymphocyte Signaling with Quantitative Phosphoproteomics.Frontiers in immunology · 2017Review
- Protein kinase D regulates positive selection of CD4Nature communications · 2016Article
- An Evolutionarily Conserved PLC-PKD-TFEB Pathway for Host Defense.Cell reports · 2016Article
- Integrative Phosphoproteomics Links IL-23R Signaling with Metabolic Adaptation in Lymphocytes.Scientific reports · 2016Article
- Protein kinase D1/2 is involved in the maturation of multivesicular bodies and secretion of exosomes in T and B lymphocytes.Cell death and differentiation · 2016Article
- Comprehensive identification of arginine methylation in primary T cells reveals regulatory roles in cell signalling.Nature communications · 2015Article
- Ubiquitylation as a Rheostat for TCR Signaling: From Targeted Approaches Toward Global Profiling.Frontiers in immunology · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The focus of the present study was to characterize the phosphoproteome of cytotoxic T cells and to explore the role of the serine threonine kinase PKD2 (Protein Kinase D2) in the phosphorylation networks of this key lymphocyte population. We used Stable Isotope Labeling of Amino acids in Culture (SILAC) combined with phosphopeptide enrichment and quantitative mass-spectrometry to determine the impact of PKD2 loss on the cytotoxic T cells phosphoproteome. We identified 15,871 phosphorylations on 3505 proteins in cytotoxic T cells. 450 phosphosites on 281 proteins were down-regulated and 300 phosphosites on 196 proteins were up-regulated in PKD2 null cytotoxic T cells. These data give valuable new insights about the protein phosphorylation networks operational in effector T cells and reveal that PKD2 regulates directly and indirectly about 5% of the cytotoxic T-cell phosphoproteome. PKD2 candidate substrates identified in this study include proteins involved in two distinct biological functions: regulation of protein sorting and intracellular vesicle trafficking, and control of chromatin structure, transcription, and translation. In other cell types, PKD substrates include class II histone deacetylases such as HDAC7 and actin regulatory proteins such as Slingshot. The current data show these are not PKD substrates in primary T cells revealing that the functional role of PKD isoforms is different in different cell lineages.
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