Evidence map›Paper›PMID 25266727›Full record

ArticleThe Journal of biological chemistry2014

Mutual exclusivity of hyaluronan and hyaluronidase in invasive group A Streptococcus.

Anna Henningham, Masaya Yamaguchi, Ramy K Aziz, Kirsten Kuipers, Cosmo Z Buffalo, Samira Dahesh, Biswa Choudhury, Jeremy Van Vleet, Yuka Yamaguchi, Lisa M Seymour and 9 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 39 citations in OpenAlex.

  1. Review
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  14. Article
  15. Playing With Fire: Proinflammatory Virulence Mechanisms of Group AFrontiers in cellular and infection microbiology · 2021
    Review
  16. Cryptic prophages within aMicrobial genomics · 2021
    Article
  17. Genetic Characterization ofInfectious microbes & diseases · 2020
    Article
  18. Article
  19. To be capsulated or not be capsulated: that is the GAS question.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019
    Article
  20. The Bacteriophages ofMicrobiology spectrum · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 7 institutions in 5 countries.

Anna HenninghamDepartment of Pediatrics, University of California San Diego, La Jolla, California 92093; School of Chemistry and Molecular Biosciences and The University of Queensland, St. Lucia, Queensland 4072, Australia; Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Masaya YamaguchiDepartment of Pediatrics, University of California San Diego, La Jolla, California 92093; Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan.
Ramy K AzizSystems Biology Research Group, University of California San Diego, La Jolla, California 92093; Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
Kirsten KuipersDepartment of Pediatrics, University of California San Diego, La Jolla, California 92093; Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Centre, 6500 HC Nijmegen, The Netherlands.
Cosmo Z BuffaloDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093.
Samira DaheshDepartment of Pediatrics, University of California San Diego, La Jolla, California 92093.
Biswa ChoudhuryGlycobiology Research and Training Center, University of California San Diego, La Jolla, California 92093.
Jeremy Van VleetGlycobiology Research and Training Center, University of California San Diego, La Jolla, California 92093.
Yuka YamaguchiDepartment of Pediatrics, University of California San Diego, La Jolla, California 92093.
Lisa M SeymourSchool of Chemistry and Molecular Biosciences and The University of Queensland, St. Lucia, Queensland 4072, Australia; Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Nouri L Ben ZakourSchool of Chemistry and Molecular Biosciences and The University of Queensland, St. Lucia, Queensland 4072, Australia; Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Lingjun HeDepartment of Mathematics and Statistics, San Diego State University, San Diego, California 92182.
Helen V SmithQueensland Health Forensic and Scientific Services, Coopers Plains, Queensland 4108, Australia.
Keith GrimwoodQueensland Children's Medical Research Institute, Herston, Queensland 4029, Australia, and.
Scott A BeatsonSchool of Chemistry and Molecular Biosciences and The University of Queensland, St. Lucia, Queensland 4072, Australia; Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Partho GhoshDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093.
Mark J WalkerSchool of Chemistry and Molecular Biosciences and The University of Queensland, St. Lucia, Queensland 4072, Australia; Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Victor NizetDepartment of Pediatrics, University of California San Diego, La Jolla, California 92093; Skaggs School of Pharmacy and Pharmaceutical Sciences, and University of California San Diego, La Jolla, California 92093; Rady Children's Hospital, San Diego, California 92123.
Jason N ColeDepartment of Pediatrics, University of California San Diego, La Jolla, California 92093; School of Chemistry and Molecular Biosciences and The University of Queensland, St. Lucia, Queensland 4072, Australia; Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland 4072, Australia,. Electronic address: jncole@ucsd.edu.
University of California, San Diego · USUniversity of Queensland · AUQueensland Children’s Medical Research Institute · AUQueensland Health · AURadboud University Medical Center · NLRady Children's Hospital-San Diego · USSan Diego State University · US

Funding

GENETIC MECHANISMS AND REGULATIONT32GM007240 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAMPTON, RANDOLPH Y. · 1985 to 2019
$23.8M
GAS Switch from Colonizing Bacterium to Invasive PathogenR01AI077780 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NIZET, VICTOR · 2008 to 2019
$3.8M
Mechanisms of the M protein in group A Streptococcus virulenceR01AI096837 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GHOSH, PARTHO, NIZET, VICTOR · 2011 to 2015
$2.7M
CATHELICIDINS--A MOLECULAR BACTERIOLOGIC APPROACHR21AI048176 · NIAID · VETERANS MEDICAL RESEARCH FDN/SAN DIEGO · PI GALLO, RICHARD L · 2000 to 2001
$420k
NIAID NIH HHS AI077780NIAID NIH HHS AI48176NIAID NIH HHS R01 AI077780NIAID NIH HHS R01 AI096837NIGMS NIH HHS T32 GM007240
6 · The paper itself

Abstract

A recent analysis of group A Streptococcus (GAS) invasive infections in Australia has shown a predominance of M4 GAS, a serotype recently reported to lack the antiphagocytic hyaluronic acid (HA) capsule. Here, we use molecular genetics and bioinformatics techniques to characterize 17 clinical M4 isolates associated with invasive disease in children during this recent epidemiology. All M4 isolates lacked HA capsule, and whole genome sequence analysis of two isolates revealed the complete absence of the hasABC capsule biosynthesis operon. Conversely, M4 isolates possess a functional HA-degrading hyaluronate lyase (HylA) enzyme that is rendered nonfunctional in other GAS through a point mutation. Transformation with a plasmid expressing hasABC restored partial encapsulation in wild-type (WT) M4 GAS, and full encapsulation in an isogenic M4 mutant lacking HylA. However, partial encapsulation reduced binding to human complement regulatory protein C4BP, did not enhance survival in whole human blood, and did not increase virulence of WT M4 GAS in a mouse model of systemic infection. Bioinformatics analysis found no hasABC homologs in closely related species, suggesting that this operon was a recent acquisition. These data showcase a mutually exclusive interaction of HA capsule and active HylA among strains of this leading human pathogen.

Indexed as

AnimalsBacterial ProteinsBase SequenceCell MembraneComputational BiologyExotoxinsFemaleGenetic Complementation TestHistidine KinaseHumansHyaluronic AcidHyaluronoglucosaminidaseIntracellular Signaling Peptides and ProteinsMiceMolecular Sequence DataNeutrophilsBacterial ProteinsCovS protein, Streptococcus pyogenesCsrR protein, Streptococcus pyogeneserythrogenic toxinExotoxinsHistidine Kinasehyaluronate lyaseHyaluronic AcidHyaluronoglucosaminidaseIntracellular Signaling Peptides and ProteinsPolysaccharide-LyasesPolysaccharidesRecombinant ProteinsRepressor ProteinsRopB protein, Rhizobium leguminosarumBacterial PathogenesisGroup A StreptococcusHyaluronanHyaluronateHyaluronate LyaseHyaluronic acid CapsuleInfectious DiseaseInvasive DiseaseNonencapsulatedStreptococcus Pyogenes (S. Pyogenes)

Identifiers

PMID25266727
PMCPMC4231703
OpenAlexW2147805408

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.