ArticleThe Journal of biological chemistry2014
Molecular mechanisms associated with xylan degradation by Xanthomonas plant pathogens.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Guardians or Gateways? The Intricate Relationship Between Plant Cell Walls and Pathogenic Xanthomonas.Molecular plant pathology · 2026Review
- Metabolic feedbacks drive population dynamics and can lead to oscillations among leaf bacteria.Nature communications · 2026Article
- Comprehensive Genomics and Functional Validation Reveal Evolutionary Insights into Xanthan Gum Biosynthesis and Pathogenesis ofJournal of agricultural and food chemistry · 2026Article
- The dual role of Xanthomonas effector XccXynB in plant cell wall deconstruction and immunity activation.Plant cell reports · 2026Article
- Enhancing functional proteins through multimodal inverse folding with ABACUS-T.Nature communications · 2025Article
- RecombinantBiotech (Basel (Switzerland)) · 2025Article
- Toad's survivability and soil microbiome alterations impacted via individual abundance.Biologia futura · 2025Article
- Single-Molecule Imaging of Wood Xylans on Surfaces and Their Interaction with GH11 Xylanase.Biomacromolecules · 2025Article
- Genomic and mutational analysis of Pseudomonas syringae pv. tagetis EB037 pathogenicity on sunflower.BMC microbiology · 2025Article
- PeSTo-Carbs: Geometric Deep Learning for Prediction of Protein-Carbohydrate Binding Interfaces.Journal of chemical theory and computation · 2024Article
- The catabolic specialization of the marine bacteriumApplied and environmental microbiology · 2024Article
- Endophytic non-pathogenicFrontiers in plant science · 2024Article
- Xylose Isomerase Depletion Enhances Virulence ofInternational journal of molecular sciences · 2023Article
- Comprehensive Genome Analysis of Cellulose and Xylan-Active CAZymes from the GenusMicrobiology spectrum · 2023Article
- Genomes of four Streptomyces strains reveal insights into putative new species and pathogenicity of scab-causing organisms.BMC genomics · 2023Article
- Root-Associated Bacteria Community Characteristics of Antarctic Plants: Deschampsia antarctica and Colobanthus quitensis-a Comparison.Microbial ecology · 2022Article
- Structural variation and spatial polysaccharide profiling of intervessel pit membranes in grapevine.Annals of botany · 2022Article
- Review
- Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors.Nature communications · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xanthomonas pathogens attack a variety of economically relevant plants, and their xylan CUT system (carbohydrate utilization with TonB-dependent outer membrane transporter system) contains two major xylanase-related genes, xynA and xynB, which influence biofilm formation and virulence by molecular mechanisms that are still elusive. Herein, we demonstrated that XynA is a rare reducing end xylose-releasing exo-oligoxylanase and not an endo-β-1,4-xylanase as predicted. Structural analysis revealed that an insertion in the β7-α7 loop induces dimerization and promotes a physical barrier at the +2 subsite conferring this unique mode of action within the GH10 family. A single mutation that impaired dimerization became XynA active against xylan, and high endolytic activity was achieved when this loop was tailored to match a canonical sequence of endo-β-1,4-xylanases, supporting our mechanistic model. On the other hand, the divergent XynB proved to be a classical endo-β-1,4-xylanase, despite the low sequence similarity to characterized GH10 xylanases. Interestingly, this enzyme contains a calcium ion bound nearby to the glycone-binding region, which is required for catalytic activity and structural stability. These results shed light on the molecular basis for xylan degradation by Xanthomonas and suggest how these enzymes synergistically assist infection and pathogenesis. Our findings indicate that XynB contributes to breach the plant cell wall barrier, providing nutrients and facilitating the translocation of effector molecules, whereas the exo-oligoxylanase XynA possibly participates in the suppression of oligosaccharide-induced immune responses.
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