ArticleThe Journal of biological chemistry2014
Hyaluronan synthase 2 protects skin fibroblasts against apoptosis induced by environmental stress.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- In vitro, ex vivo, instrumental and clinical evaluation of a topical cream on the signs of periorbital ageing.International journal of cosmetic science · 2025Trial
- Fibroblast-derived CCL2 orchestrates immune responses and defends against Staphylococcus aureus skin infection.Cellular & molecular immunology · 2026Article
- Hyaluronic Acid-like Skin Plumping and Radiance Benefits of aMarine drugs · 2026Article
- Beneficial Effects ofJournal of microbiology and biotechnology · 2026Article
- Fermented Plant-Derived Phytoconstituents in Biocosmetics: Pharmacological Enhancements and Applications.Clinical, cosmetic and investigational dermatology · 2026Review
- Article
- Targeting hyaluronan synthesis enhances the therapeutic effectiveness of biologics in inflammatory bowel disease.JCI insight · 2025Article
- β-hydroxybutyrate induces endoplasmic reticulum stress in bovine cumulus cells during oocyte maturationAnimal reproduction · 2025Article
- An Advanced Combinatorial System fromPharmaceuticals (Basel, Switzerland) · 2024Article
- Piceatannol enhances hyaluronic acid synthesis through SIRT1-Mediated HAS2 upregulation in human dermal fibroblasts.Biochemistry and biophysics reports · 2024Article
- The Superior Effect of Radiofrequency With Targeted Ultrasound for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis: A Pilot Preclinical Study.Aesthetic surgery journal. Open forum · 2024Article
- The Effects of Helichrysum italicum Extract on the Extracellular Matrix of the Skin.Acta medica academica · 2023Article
- BCL2 is a major regulator of haploidy maintenance in murine embryonic stem cells.Cell proliferation · 2023Article
- Transforming Growth Factor-β Receptor-Mediated, p38 Mitogen-Activated Protein Kinase-Dependent Signaling Drives Enhanced Myofibroblast Differentiation during Skin Wound Healing in Mice Lacking Hyaluronan Synthases 1 and 3.The American journal of pathology · 2022Article
- Hyaluronan nanoscale clustering and Hyaluronan synthase 2 expression are linked to the invasion of child fibroblasts and infantile fibrosarcoma in vitro and in vivo.Scientific reports · 2022Article
- 3'UTR shortening of HAS2 promotes hyaluronan hyper-synthesis and bioenergetic dysfunction in pulmonary hypertension.Matrix biology : journal of the International Society for Matrix Biology · 2022Article
- In Vitro and Clinical Evaluation of Cannabigerol (CBG) Produced via Yeast Biosynthesis: A Cannabinoid with a Broad Range of Anti-Inflammatory and Skin Health-Boosting Properties.Molecules (Basel, Switzerland) · 2022Article
- Matters of size: Roles of hyaluronan in CNS aging and disease.Ageing research reviews · 2021Review
- Nanomaterials in Skin Regeneration and Rejuvenation.International journal of molecular sciences · 2021Review
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A balanced turnover of dermal fibroblasts is crucial for structural integrity and normal function of the skin. During recovery from environmental injury (such as UV exposure and physical wounding), apoptosis is an important mechanism regulating fibroblast turnover. We are interested in the role that hyaluronan (HA), an extracellular matrix molecule synthesized by HA synthase enzymes (Has), plays in regulating apoptosis in fibroblasts. We previously reported that Has1 and Has3 double knock-out (Has1/3 null) mice show accelerated wound closure and increased numbers of fibroblasts in the dermis. In the present study, we report that HA levels and Has2 mRNA expression are higher in cultured Has1/3 null primary skin fibroblasts than in wild type (WT) cells. Apoptosis induced by two different environmental stressors, UV exposure and serum starvation (SS), was reduced in the Has1/3 null cells. Hyaluronidase, added to cultures to remove extracellular HA, surprisingly had no effect upon apoptotic susceptibility to UVB or SS. However, cells treated with 4-methylumbelliferone to inhibit HA synthesis were sensitized to apoptosis induced by SS or UVB. When fibroblasts were transfected with Has2-specific siRNA that lowered Has2 mRNA and HA levels by 90%, both Has1/3 null and WT cells became significantly more sensitive to apoptosis. The exogenous addition of high molecular weight HA failed to reverse this effect. We conclude that Has1/3 null skin fibroblasts (which have higher levels of Has2 gene expression) are resistant to stress-induced apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.