ArticlePlant physiology2014
The role of plasma membrane aquaporins in regulating the bundle sheath-mesophyll continuum and leaf hydraulics.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 122 citations in OpenAlex.
- Hydraulic Signaling in Plants: From Physical Perturbation to Distributed Perception and Context-Dependent Decoding.Plants (Basel, Switzerland) · 2026Review
- Rapid dehydration drives a nondiffusional drop in CThe New phytologist · 2026Article
- Tomato in the spotlight: light regulation of whole-plant physiology.Journal of experimental botany · 2025Review
- Lost in translation: What we have learned from attributes that do not translate from Arabidopsis to other plants.The Plant cell · 2025Review
- Localized measurements of water potential reveal large loss of conductance in living tissues of maize leaves.Plant physiology · 2024Article
- Leaf hydraulic maze: Abscisic acid effects on bundle sheath, palisade, and spongy mesophyll conductance.Plant physiology · 2023Article
- Anatomical determinants of gas exchange and hydraulics vary with leaf shape in soybean.Annals of botany · 2023Article
- A high-throughput yeast approach to characterize aquaporin permeabilities: Profiling the Arabidopsis PIP aquaporin sub-family.Frontiers in plant science · 2023Article
- Safety-efficiency tradeoffs? Correlations of photosynthesis, leaf hydraulics, and dehydration tolerance across species.Oecologia · 2022Article
- Out of the blue: Phototropins of the leaf vascular bundle sheath mediate the regulation of leaf hydraulic conductance by blue light.The Plant cell · 2022Article
- Evaporative flux method of leaf hydraulic conductance estimation: sources of uncertainty and reporting format recommendation.Plant methods · 2022Article
- Experimental and conceptual approaches to root water transport.Plant and soil · 2022Review
- Optimizing Crop Water Use for Drought and Climate Change Adaptation Requires a Multi-Scale Approach.Frontiers in plant science · 2022Review
- Differential regulation of drought stress by biological membrane transporters and channels.Plant cell reports · 2021Review
- Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Breeding for sustainable oilseed crop yield and quality in a changing climate.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Efficient Regulation of COFrontiers in plant science · 2021Article
- Versatile Roles of Aquaporins in Plant Growth and Development.International journal of molecular sciences · 2020Review
- Vascular Bundles Mediate Systemic Reactive Oxygen Signaling during Light Stress.The Plant cell · 2020Article
- Exogenous melatonin alleviates PEG-induced short-term water deficiency in maize by increasing hydraulic conductance.BMC plant biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our understanding of the cellular role of aquaporins (AQPs) in the regulation of whole-plant hydraulics, in general, and extravascular, radial hydraulic conductance in leaves (K(leaf)), in particular, is still fairly limited. We hypothesized that the AQPs of the vascular bundle sheath (BS) cells regulate K(leaf). To examine this hypothesis, AQP genes were silenced using artificial microRNAs that were expressed constitutively or specifically targeted to the BS. MicroRNA sequences were designed to target all five AQP genes from the PLASMA MEMBRANE-INTRINSIC PROTEIN1 (PIP1) subfamily. Our results show that the constitutively silenced PIP1 (35S promoter) plants had decreased PIP1 transcript and protein levels and decreased mesophyll and BS osmotic water permeability (P(f)), mesophyll conductance of CO2, photosynthesis, K(leaf), transpiration, and shoot biomass. Plants in which the PIP1 subfamily was silenced only in the BS (SCARECROW:microRNA plants) exhibited decreased mesophyll and BS Pf and decreased K(leaf) but no decreases in the rest of the parameters listed above, with the net result of increased shoot biomass. We excluded the possibility of SCARECROW promoter activity in the mesophyll. Hence, the fact that SCARECROW:microRNA mesophyll exhibited reduced P(f), but not reduced mesophyll conductance of CO2, suggests that the BS-mesophyll hydraulic continuum acts as a feed-forward control signal. The role of AQPs in the hierarchy of the hydraulic signal pathway controlling leaf water status under normal and limited-water conditions is discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.