ArticleJournal of bacteriology2014
Characterization of p-hydroxycinnamate catabolism in a soil Actinobacterium.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- A new amidohydrolase and β-oxidation-like pathway for piperine catabolism in soil actinomycetes.The Journal of biological chemistry · 2025Article
- Kraft lignin decomposition by lignin-derived aromatic compound degrader Rhodococcus sp. DK17.World journal of microbiology & biotechnology · 2025Article
- Biodetoxification of Lignocellulose Hydrolysate for Direct Use in Succinic Acid Production.Biodesign research · 2024Article
- MarR Family Transcriptional Regulators and Their Roles in Plant-Interacting Bacteria.Microorganisms · 2023Review
- Characterization of Two Marine Lignin-Degrading Consortia and the Potential Microbial Lignin Degradation Network in Nearshore Regions.Microbiology spectrum · 2023Article
- Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering.The New phytologist · 2023Article
- Identification of a Phylogenetically Divergent Vanillate O-Demethylase fromMicroorganisms · 2022Article
- Bacterial catabolism of acetovanillone, a lignin-derived compound.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Catabolic System of Acetovanillone and Acetosyringone inApplied and environmental microbiology · 2022Article
- Applying biochemical and structural characterization of hydroxycinnamate catabolic enzymes from soil metagenome for lignin valorization strategies.Applied microbiology and biotechnology · 2022Article
- Aerobic Methoxydotrophy: Growth on Methoxylated Aromatic Compounds byFrontiers in microbiology · 2022Article
- Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification.Scientific reports · 2021Article
- Aspergillus niger uses the peroxisomal CoA-dependent β-oxidative genes to degrade the hydroxycinnamic acids caffeic acid, ferulic acid, and p-coumaric acid.Applied microbiology and biotechnology · 2021Article
- The Operon Encoding Hydrolytic Dehalogenation of 4-Chlorobenzoate Is Transcriptionally Regulated by the TetR-Type Repressor FcbR and Its Ligand 4-Chlorobenzoyl Coenzyme A.Applied and environmental microbiology · 2021Article
- Bacterial Transformation of Aromatic Monomers in Softwood Black Liquor.Frontiers in microbiology · 2021Article
- Characterization of alkylguaiacol-degrading cytochromes P450 for the biocatalytic valorization of lignin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Functional genomic analysis of bacterial lignin degraders: diversity in mechanisms of lignin oxidation and metabolism.Applied microbiology and biotechnology · 2020Review
- IpdE1-IpdE2 Is a Heterotetrameric Acyl Coenzyme A Dehydrogenase That Is Widely Distributed in Steroid-Degrading Bacteria.Biochemistry · 2020Article
- Horizontal transfer of a pathway for coumarate catabolism unexpectedly inhibits purine nucleotide biosynthesis.Molecular microbiology · 2019Article
- Passive membrane transport of lignin-related compounds.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
p-Hydroxycinnamates, such as ferulate and p-coumarate, are components of plant cell walls and have a number of commercial applications. Rhodococcus jostii RHA1 (RHA1) catabolizes ferulate via vanillate and the β-ketoadipate pathway. Here, we used transcriptomics to identify genes in RHA1 that are upregulated during growth on ferulate versus benzoate. The upregulated genes included three transcriptional units predicted to encode the uptake and β-oxidative deacetylation of p-hydroxycinnamates: couHTL, couNOM, and couR. Neither ΔcouL mutants nor ΔcouO mutants grew on p-hydroxycinnamates, but they did grow on vanillate. Among several p-hydroxycinnamates, CouL catalyzed the thioesterification of p-coumarate and caffeate most efficiently (k(cat)/K(m) = ∼ 400 mM(-1) s(-1)). p-Coumarate was also RHA1's preferred growth substrate, suggesting that CouL is a determinant of the pathway's specificity. CouL did not catalyze the activation of sinapate, in similarity to two p-coumaric acid:coenzyme A (CoA) ligases from plants, and contains the same bulged loop that helps determine substrate specificity in the plant homologues. The couO mutant accumulated 4-hydroxy-3-methoxyphenyl-β-ketopropionate in the culture supernatant when incubated with ferulate, supporting β-oxidative deacetylation. This phenotype was not complemented with a D257N variant of CouO, consistent with the predicted role of Asp257 as a metal ligand in this amidohydrolase superfamily member. These data suggest that CouO functionally replaces the β-ketothiolase and acyl-CoA thioesterase that occur in canonical β-oxidative pathways. Finally, the transcriptomics data suggest the involvement of two distinct formaldehyde detoxification pathways in vanillate catabolism and identify a eugenol catabolic pathway. The results of this study augment our understanding of the bacterial catabolism of aromatics from renewable feedstocks.
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