ArticleLangmuir : the ACS journal of surfaces and colloids2014
Recombinant protein-stabilized monodisperse microbubbles with tunable size using a valve-based microfluidic device.
Article in Langmuir : the ACS journal of surfaces and colloids, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Influence of Pluronic F68 on Size Stability and Acoustic Behavior of Monodisperse Phospholipid-Coated Microbubbles Produced at Room Temperature.ACS applied materials & interfaces · 2025Article
- A microfluidic platform for the synthesis of polymer and polymer-protein-based protocells.The European physical journal. E, Soft matter · 2024Article
- High-Speed Generation of Microbubbles with Constant Cumulative Production in a Glass Capillary Microfluidic Bubble Generator.Micromachines · 2024Article
- Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation.iScience · 2023Article
- Microbubbles Stabilized by Protein Shell: From Pioneering Ultrasound Contrast Agents to Advanced Theranostic Systems.Pharmaceutics · 2022Review
- Protein-Based Encapsulation Strategies: Toward Micro- and Nanoscale Carriers with Increased Functionality.Small science · 2022Article
- Scaling up the throughput of microfluidic droplet-based materials synthesis: A review of recent progress and outlook.Applied physics reviews · 2021Review
- Requirement and Development of Hydrogel Micromotors towards Biomedical Applications.Research (Washington, D.C.) · 2020Review
- Formulation and Characterization of Chemically Cross-linked Microbubble Clusters.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Engineering the Echogenic Properties of Microfluidic Microbubbles Using Mixtures of Recombinant Protein and Amphiphilic Copolymers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Engineering Theranostic Microbubbles Using Microfluidics for Ultrasound Imaging and Therapy: A Review.Ultrasound in medicine & biology · 2018Review
- Liter-scale production of uniform gas bubbles via parallelization of flow-focusing generators.Lab on a chip · 2017Article
- Microfluidic manufacture of rt-PA -loaded echogenic liposomes.Biomedical microdevices · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Microbubbles are used as contrast enhancing agents in ultrasound sonography and more recently have shown great potential as theranostic agents that enable both diagnostics and therapy. Conventional production methods lead to highly polydisperse microbubbles, which compromise the effectiveness of ultrasound imaging and therapy. Stabilizing microbubbles with surfactant molecules that can impart functionality and properties that are desirable for specific applications would enhance the utility of microbubbles. Here we generate monodisperse microbubbles with a large potential for functionalization by combining a microfluidic method and recombinant protein technology. Our microfluidic device uses an air-actuated membrane valve that enables production of monodisperse microbubbles with narrow size distribution. The size of microbubbles can be precisely tuned by dynamically changing the dimension of the channel using the valve. The microbubbles are stabilized by an amphiphilic protein, oleosin, which provides versatility in controlling the functionalization of microbubbles through recombinant biotechnology. We show that it is critical to control the composition of the stabilizing agents to enable formation of highly stable and monodisperse microbubbles that are echogenic under ultrasound insonation. Our protein-shelled microbubbles based on the combination of microfluidic generation and recombinant protein technology provide a promising platform for ultrasound-related applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.