ArticlePloS one2014
Dynamic autophosphorylation of mps1 kinase is required for faithful mitotic progression.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mitotic error correction and the spindle assembly checkpoint: a tension-filled relationship.Cell cycle (Georgetown, Tex.) · 2026Review
- The cytotoxicity of microcystin-LR: ultrastructural and functional damage of cells.Archives of toxicology · 2024Review
- PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression.Scientific reports · 2021Article
- Mps1 dimerization and multisite interactions with Ndc80 complex enable responsive spindle assembly checkpoint signaling.Journal of molecular cell biology · 2020Article
- A common molecular mechanism underlies the role of Mps1 in chromosome biorientation and the spindle assembly checkpoint.EMBO reports · 2020Article
- Mitotic checkpoint kinase Mps1/TTK predicts prognosis of colon cancer patients and regulates tumor proliferation and differentiation via PKCα/ERK1/2 and PI3K/Akt pathway.Medical oncology (Northwood, London, England) · 2019Article
- Checkpoint signaling and error correction require regulation of the MPS1 T-loop by PP2A-B56.The Journal of cell biology · 2019Article
- Autophosphorylation is sufficient to release Mps1 kinase from native kinetochores.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling.The Journal of cell biology · 2019Article
- Leader of the SAC: molecular mechanisms of Mps1/TTK regulation in mitosis.Open biology · 2018Review
- Playing polo during mitosis: PLK1 takes the lead.Oncogene · 2017Review
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.Nature communications · 2015Article
- Dynamic localization of Mps1 kinase to kinetochores is essential for accurate spindle microtubule attachment.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mitotic Checkpoint Kinase Mps1 Has a Role in Normal Physiology which Impacts Clinical Utility.PloS one · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spindle assembly checkpoint (SAC) is a surveillance mechanism monitoring cell cycle progression, thus ensuring accurate chromosome segregation. The conserved mitotic kinase Mps1 is a key component of the SAC. The human Mps1 exhibits comprehensive phosphorylation during mitosis. However, the related biological relevance is largely unknown. Here, we demonstrate that 8 autophosphorylation sites within the N-terminus of Mps1, outside of the catalytic domain, are involved in regulating Mps1 kinetochore localization. The phospho-mimicking mutant of the 8 autophosphorylation sites impairs Mps1 localization to kinetochore and also affects the kinetochore recruitment of BubR1 and Mad2, two key SAC effectors, subsequently leading to chromosome segregation errors. Interestingly, the non-phosphorylatable mutant of the 8 autophosphorylation sites enhances Mps1 kinetochore localization and delays anaphase onset. We further show that the Mps1 phospho-mimicking and non-phosphorylatable mutants do not affect metaphase chromosome congression. Thus, our results highlight the importance of dynamic autophosphorylation of Mps1 in regulating accurate chromosome segregation and ensuring proper mitotic progression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.