ArticlePloS one2014
Incidence of and factors associated with false positives in laboratory diagnosis of norovirus infection by amplification of the RNA-dependent RNA polymerase gene.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Development of a new TaqMan real-time PCR for detecting Psittacine adenovirus 1 (PsAdV-1).BMC veterinary research · 2026Article
- Detection and Sequencing of Multiple Human Norovirus Genotypes from Imported Frozen Raspberries Linked to Outbreaks in the Province of Quebec, Canada, in 2017.Food and environmental virology · 2022Article
- Advanced detection strategies for cardiotropic virus infection in a cohort study of heart failure patients.Laboratory investigation; a journal of technical methods and pathology · 2022Article
- Thermostable Heptaplex PCR Assay for the Detection of Six Respiratory Bacterial Pathogens.Diagnostics (Basel, Switzerland) · 2021Article
- Article
- Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification inBurns & trauma · 2020Article
- Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses.Scientific reports · 2017Article
- Observational
- Surveillance of Food- and Smear-Transmitted Pathogens in European Soldiers with Diarrhea on Deployment in the Tropics: Experience from the European Union Training Mission (EUTM) Mali.BioMed research international · 2015Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConventional reverse transcription-polymerase chain reaction (RT-PCR) amplification of the RNA-dependent RNA polymerase (RdRp) gene remains a used method for the rapid detection of norovirus (NV) in clinical laboratories. The incidence of and factors associated with false positives in this assay have not been previously evaluated. METHODS/PRINCIPAL
findingsAfter an NV outbreak caused by the GII.4 Sydney strain in 2012, we reanalysed 250 stool samples positive for NV by RdRp gene detection. True positives were confirmed in 154 (61.6%) samples by successful amplification and sequencing confirmation of the viral protein 1 gene. Of the remaining 96 samples that underwent RT-PCR for the RdRp gene, 34 samples yielded PCR products of the expected length. However, the sequences of the amplicons belonged to the human genome, with 91-97% matched nucleotide sequences, indicating false positives. Multivariate analysis of the clinical features of the patients identified a positive stool culture for bacteria (adjusted odds ratio [aOR] 9.07, 95% adjusted confidence interval [aCI] 2.17-37.92, P = .003) and the use of parenteral antibiotics (aOR 5.55, 95% aCI 1.21-24.73, P = .027) as significant and independent factors associated with false positives.
conclusionConventional RT-PCR targeting the RdRp gene of NV can lead to false positives in patients with bacterial enterocolitis by incidental amplification of DNA from a human source.
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