ArticleMolecular cytogenetics2014
Smoking-induced chromosomal segregation anomalies identified by FISH analysis of sperm.
Article in Molecular cytogenetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Pooled it
- Comparative Assessment of Manual Versus Automated Semen Analysis and the Role of Novel Biomarker Calprotectin in Screening for Male Factor Infertility.Iranian journal of pathology · 2026Article
- Lifestyle implications of the paradox and management of oxidative stress in sperm.The Journal of physiology · 2026Review
- The combined effect between environmental exposure and oxidative stress-related susceptible gene polymorphisms on human semen quality.Journal of assisted reproduction and genetics · 2025Article
- Effects of marijuana and tobacco on male fertility and their relationship to genetic variation of mitochondrial cytochrome C oxidase genes.Scientific reports · 2025Article
- The mutagenic effect of tobacco smoke on male fertility.Environmental science and pollution research international · 2022Review
- Exposures during the prepuberty period and future offspring's health: evidence from human cohort studies†.Biology of reproduction · 2021Review
- Preventive Healthcare and Management for Acute Lymphoblastic Leukaemia in Adults: Case Report and Literature Review.Healthcare (Basel, Switzerland) · 2021Article
- No influence of smoking status on the performance of urine markers for the detection of bladder cancer.Journal of cancer research and clinical oncology · 2018Article
- Smoking and Male Infertility: An Evidence-Based Review.The world journal of men's health · 2015Review
- Models of germ cell development and their application for toxicity studies.Environmental and molecular mutagenesis · 2015Review
- Nuclear and mitochondrial DNA alterations in newborns with prenatal exposure to cigarette smoke.International journal of environmental research and public health · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNumerical chromosome aberrations in gametes are directly related to infertility and aneuploid embryos. Previous studies have shown that toxic substances from cigarette smoke induce structural and numerical chromosomal aberrations in vitro and could potentially increase levels of aneusomy in sperm. Moreover, increased levels of aneusomy in sperm are correlated with low implantation rates, spontaneous abortions and fetal losses. Studies of chromosome 3 in sperm suggest it may be more prone to segregation anomalies than other autosomes, but there has been no systematic investigation of the incidence of disomy for chromosome 3 in sperm derived from donor male smokers. The objective of this study was to use FISH to evaluate the influence of smoking on the levels of disomy for chromosomes X and Y, and to determine whether disomy levels for chromosome 3 were elevated in sperm derived from male smokers.
resultsFISH analysis was used to evaluate the frequency of disomies of chromosomes 3, X, and Y in sperm of 10 smokers, compared to a control group of 7 non-smoking fertile men. All the subjects presented a normal somatic karyotype. There was a significant increase in the overall frequency of disomies in sperm derived from the smoking group (P< 0.0001). When each chromosome pair was analyzed individually, disomy of chromosome 3 in smokers was found to be more than twice that observed in the matched non-smoker control group. In addition we observed a higher frequencies of disomy of the X and Y chromosomes, indicating elevated levels of diploidy in the sperm from the smoking group.
conclusionsIn this study we have shown that chromosome 3 may be susceptible to smoking-related segregation anomalies. Our results also suggest that errors can occur in both meiosis I and II, confirming the emerging literature that the male meiotic process may generally be affected by the genotoxic damage from tobacco use. Collectively, these findings provide additional evidence for enhancing tobacco control measures, and suggest that FISH analysis of chromosome 3 in sperm may be useful for monitoring smoking-induced segregation damage as part of the evaluation of infertile males.
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Registered trials
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