ArticleCurrent biology : CB2014
Kinesin-12 Kif15 targets kinetochore fibers through an intrinsic two-step mechanism.
Article in Current biology : CB, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 55 citations in OpenAlex.
- Microtubule depolymerization at kinetochores restricts anaphase spindle elongation.Nature chemical biology · 2026Article
- KIF15 (Kinesin-12): molecular biology, physiological functions, and roles in disease.Journal of cancer research and clinical oncology · 2026Review
- In vitro reconstitution defines the mechanistic basis of HSET motor activity regulation by IntraFlagellar Transport proteins.Communications biology · 2026Article
- The Chromatin Protein CFDP1 Activates TPX2 and Promotes Chromosomal Microtubule Nucleation and Spindle Assembly.International journal of molecular sciences · 2026Article
- Kinesin-12 POK2 polarization is a prerequisite for a fully functional division site and aids cell plate positioning.Nature communications · 2025Article
- Antiparallel microtubule bundling supports KIF15-driven mitotic spindle assembly.Molecular biology of the cell · 2024Article
- Synergy between inhibitors of two mitotic spindle assembly motors undermines an adaptive response.Molecular biology of the cell · 2022Article
- Kinesin KIF15 regulates tubulin acetylation and spindle assembly checkpoint in mouse oocyte meiosis.Cellular and molecular life sciences : CMLS · 2022Article
- Acentrosomal spindle assembly and maintenance inMolecular biology of the cell · 2022Article
- Drug resistance dependent on allostery: A P-loop rigor Eg5 mutant exhibits resistance to allosteric inhibition by STLC.Frontiers in oncology · 2022Article
- Chemical Biology of Mitotic Spindle Assembly Motors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15.Molecular biology of the cell · 2021Article
- Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding.Science advances · 2021Article
- KINESIN-12E regulates metaphase spindle flux and helps control spindle size in Arabidopsis.The Plant cell · 2021Article
- Run length distribution of dimerized kinesin-3 molecular motors: comparison with dimeric kinesin-1.Scientific reports · 2019Article
- Plant Kinesin-12: Localization Heterogeneity and Functional Implications.International journal of molecular sciences · 2019Review
- Review
- Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15.The Journal of cell biology · 2019Article
- Dual inhibition of Kif15 by oxindole and quinazolinedione chemical probes.Bioorganic & medicinal chemistry letters · 2019Article
- The Spindle: Integrating Architecture and Mechanics across Scales.Trends in cell biology · 2018Review
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Proteins that recognize and act on specific subsets of microtubules (MTs) enable the varied functions of the MT cytoskeleton. We recently discovered that Kif15 localizes exclusively to kinetochore fibers (K-fibers) or bundles of kinetochore-MTs within the mitotic spindle. It is currently speculated that the MT-associated protein TPX2 loads Kif15 onto spindle MTs, but this model has not been rigorously tested. Here, we show that Kif15 accumulates on MT bundles as a consequence of two inherent biochemical properties. First, Kif15 is self-repressed by its C terminus. Second, Kif15 harbors a nonmotor MT-binding site, enabling dimeric Kif15 to crosslink and slide MTs. Two-MT binding activates Kif15, resulting in its accumulation on and motility within MT bundles but not on individual MTs. We propose that Kif15 targets K-fibers via an intrinsic two-step mechanism involving molecular unfolding and two-MT binding. This work challenges the current model of Kif15 regulation and provides the first account of a kinesin that specifically recognizes a higher-order MT array.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.