ArticleBiomaterials2014
Micro-scale and meso-scale architectural cues cooperate and compete to direct aligned tissue formation.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Bridging the missing middle in osseointegration: meso-scale topography between macro design and microroughness.International journal of implant dentistry · 2025Pooled it
- Hierarchical piezoelectric conduit coordinates bioelectric cues and microRNA regulation for functional neurological regeneration.Materials today. Bio · 2026Article
- Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell-Material Interactions.Bioengineering (Basel, Switzerland) · 2026Article
- Development of a 3D Human Colon Model Along with Bioelectronics for the Induction and Monitoring of Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- An ingenious design from nature to accelerate the repair of long-bone critical defects: the longitudinal tubular transverse interconnection structure of deer antlers.Materials today. Bio · 2025Article
- Controlled Stiffness of Direct-Write, Near-Field Electrospun Gelatin Fibers Generates Differences in Tenocyte Morphology and Gene Expression.Journal of biomechanical engineering · 2024Article
- Multiscale mechanical characterization and computational modeling of fibrin gels.Acta biomaterialia · 2023Article
- Suspension bath bioprinting and maturation of anisotropic meniscal constructs.Biofabrication · 2023Article
- A Micro-Scale Non-Linear Finite Element Model to Optimize the Mechanical Behavior of Bioprinted Constructs.3D printing and additive manufacturing · 2022Article
- From Soft to Hard Biomimetic Materials: Tuning Micro/Nano-Architecture of Scaffolds for Tissue Regeneration.Micromachines · 2022Review
- Tissue Engineering Meets Nanotechnology: Molecular Mechanism Modulations in Cornea Regeneration.Micromachines · 2021Review
- Cellular Geometry Sensing at Different Length Scales and its Implications for Scaffold Design.Materials (Basel, Switzerland) · 2020Review
- Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration.International journal of oral science · 2020Review
- Mechanosensing of Mechanical Confinement by Mesenchymal-Like Cells.Frontiers in physiology · 2020Review
- Cell migration: implications for repair and regeneration in joint disease.Nature reviews. Rheumatology · 2019Review
- TRPV4-mediated calcium signaling in mesenchymal stem cells regulates aligned collagen matrix formation and vinculin tension.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Biomaterials: Foreign Bodies or Tuners for the Immune Response?International journal of molecular sciences · 2019Review
- Synthetic scaffolds for musculoskeletal tissue engineering: cellular responses to fiber parameters.NPJ Regenerative medicine · 2019Review
- Mesoscale substrate curvature overrules nanoscale contact guidance to direct bone marrow stromal cell migration.Journal of the Royal Society, Interface · 2018Article
- Contact guidance and collective migration in the advancing epithelial monolayer.Connective tissue research · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Tissue and biomaterial microenvironments provide architectural cues that direct important cell behaviors including cell shape, alignment, migration, and resulting tissue formation. These architectural features may be presented to cells across multiple length scales, from nanometers to millimeters in size. In this study, we examined how architectural cues at two distinctly different length scales, "micro-scale" cues on the order of ∼1-2 μm, and "meso-scale" cues several orders of magnitude larger (>100 μm), interact to direct aligned neo-tissue formation. Utilizing a micro-photopatterning (μPP) model system to precisely arrange cell-adhesive patterns, we examined the effects of substrate architecture at these length scales on human mesenchymal stem cell (hMSC) organization, gene expression, and fibrillar collagen deposition. Both micro- and meso-scale architectures directed cell alignment and resulting tissue organization, and when combined, meso cues could enhance or compete against micro-scale cues. As meso boundary aspect ratios were increased, meso-scale cues overrode micro-scale cues and controlled tissue alignment, with a characteristic critical width (∼500 μm) similar to boundary dimensions that exist in vivo in highly aligned tissues. Meso-scale cues acted via both lateral confinement (in a cell-density-dependent manner) and by permitting end-to-end cell arrangements that yielded greater fibrillar collagen deposition. Despite large differences in fibrillar collagen content and organization between μPP architectural conditions, these changes did not correspond with changes in gene expression of key matrix or tendon-related genes. These findings highlight the complex interplay between geometric cues at multiple length scales and may have implications for tissue engineering strategies, where scaffold designs that incorporate cues at multiple length scales could improve neo-tissue organization and resulting functional outcomes.
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Registered trials
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