Evidence map›Paper›PMID 25262410›Full record

ArticleColloids and surfaces. B, Biointerfaces2014

Bi-ligand surfaces with oriented and patterned protein for real-time tracking of cell migration.

Varadraj N Vernekar, Charles S Wallace, Mina Wu, Joshua T Chao, Shannon K O'Connor, Aimee Raleigh, Xiaji Liu, Jason M Haugh, William M Reichert

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Colloids and surfaces. B, Biointerfaces, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Quantitative analysis of B-lymphocyte migration directed by CXCL13.Integrative biology : quantitative biosciences from nano to macro · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Varadraj N VernekarDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, United States.
Charles S WallaceDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, United States.
Mina WuDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, United States.
Joshua T ChaoDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, United States.
Shannon K O'ConnorDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, United States.
Aimee RaleighDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, United States.
Xiaji LiuDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, United States.
Jason M HaughDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, United States.
William M ReichertDepartment of Biomedical Engineering, Duke University, Durham, NC 27708, United States. Electronic address: reichert@duke.edu.

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007171 · NIGMS · DUKE UNIVERSITY · PI KONTOS, CHRISTOPHER D · 1985 to 2021
$31.2M
BIOSENSOR BIOCOMPATIBILITYR01DK054932 · NIDDK · DUKE UNIVERSITY · PI REICHERT, WILLIAM MONTGOMERY · 1999 to 2013
$4.2M
Self Healing BiomaterialsR21EB013874 · NIBIB · DUKE UNIVERSITY · PI REICHERT, WILLIAM MONTGOMERY · 2011 to 2012
$383k
NIAID NIH HHS HHSN272201000053CNIBIB NIH HHS R21 EB013874NIDDK NIH HHS R01 DK054932NIDDK NIH HHS R01-DK-054932NIGMS NIH HHS T32 GM007171
6 · The paper itself

Abstract

A bioactive platform for the quantitative observation of cell migration is presented by (1) presenting migration factors in a well-defined manner on 2-D substrates, and (2) enabling continuous cell tracking. Well-defined substrate presentation is achieved by correctly orienting immobilized proteins (chemokines and cell adhesion molecules), such that the active site is accessible to cell surface receptors. A thiol-terminated self-assembled monolayer on a silica slide was used as a base substrate for subsequent chemistry. The thiol-terminated surface was converted to an immobilized metal ion surface using a maleimido-nitrilotriacetic acid (NTA) cross-linker that bound Histidine-tagged recombinant proteins on the surface with uniform distribution and specific orientation. This platform was used to study the influence of surface-immobilized chemokine SDF-1α and cell adhesion molecule ICAM-1 on murine splenic B lymphocyte migration. While soluble SDF-1α induced trans-migration in a Boyden Chamber type chemotaxis assay, immobilized SDF-1α alone did not elicit significant surface-migration on our test-platform surface. Surface-immobilized cell adhesion protein, ICAM-1, in conjunction with activation enabled migration of this cell type on our surface. Controlled exposure to UV light was used to produce stable linear gradients of His-tagged recombinant SDF-1α co-immobilized with ICAM-1 following our surface chemistry approach. XPS and antibody staining showed defined gradients of outwardly oriented SDF-1α active sites. This test platform can be especially valuable for investigators interested in studying the influence of surface-immobilized factors on cell behavior and may also be used as a cell migration enabling platform for testing the effects of various diffusible agents.

Indexed as

AnimalsB-LymphocytesCell MovementCells, CulturedChemokine CXCL12Intercellular Adhesion Molecule-1MiceMice, TransgenicUltraviolet RaysChemokine CXCL12Intercellular Adhesion Molecule-1B lymphocyteCell adhesion moleculeChemokineChemotaxisSurface gradientsSurface immobilization

Identifiers

PMID25262410
PMCPMC4259856

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.