ArticleApplied and environmental microbiology2014
Microfluidic quantitative PCR for simultaneous quantification of multiple viruses in environmental water samples.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Risk management of viral infectious diseases in wastewater reclamation and reuse: Review.Environment international · 2016Pooled it
- Comparative evaluation of two high-throughput qPCR systems for environmental and clinical microbiology applications.Applied and environmental microbiology · 2026Article
- Exploring the Pathogen Profiles of Ancient Feces.bioRxiv : the preprint server for biology · 2025Article
- Targeted pathogen profiling of ancient feces reveals common enteric infections in the Rio Zape Valley, 725-920 CE.PloS one · 2025Article
- Rotavirus in Water Environments: A Systematic Review and Meta-Analysis.Environmental health insights · 2024Review
- Development of a Nanoplate-Based Digital PCR Test Method for Quantitative Detection of Human Adenovirus DNA.Infectious diseases & clinical microbiology · 2023Article
- High-Throughput Microfluidic Quantitative PCR Platform for the Simultaneous Quantification of Pathogens, Fecal Indicator Bacteria, and Microbial Source Tracking Markers.ACS ES&T water · 2023Article
- Temporal assessment of N-cycle microbial functions in a tropical agricultural soil using gene co-occurrence networks.PloS one · 2023Article
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Detection of Emerging Pollutants Using Aptamer-Based Biosensors: Recent Advances, Challenges, and Outlook.Biosensors · 2022Review
- Sewage surveillance for SARS-CoV-2: Molecular detection, quantification, and normalization factors.Current opinion in environmental science & health · 2022Review
- Article
- Roadmap for Managing SARS-CoV-2 and Other Viruses in the Water Environment for Public Health.Engineering (Beijing, China) · 2022Review
- Maize germplasm chronosequence shows crop breeding history impacts recruitment of the rhizosphere microbiome.The ISME journal · 2021Article
- Quantification and Trends of Rotavirus and Enterovirus in Untreated Sewage Using Reverse Transcription Droplet Digital PCR.Food and environmental virology · 2021Article
- Coronavirus (SARS-CoV-2) in the environment: Occurrence, persistence, analysis in aquatic systems and possible management.The Science of the total environment · 2021Review
- SARS-CoV-2 in hospital wastewater during outbreak of COVID-19: A review on detection, survival and disinfection technologies.The Science of the total environment · 2021Review
- Distribution and Antibiotic Resistance Profiles ofGeoHealth · 2021Article
- Wastewater and public health: the potential of wastewater surveillance for monitoring COVID-19.Current opinion in environmental science & health · 2020Review
- Viral indicators for tracking domestic wastewater contamination in the aquatic environment.Water research · 2020Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sapovirus, and hepatitis A and E viruses. Murine norovirus and mengovirus were also quantified in our MFQPCR system as a sample processing control and an internal amplification control, respectively. River water contaminated with effluents from a wastewater treatment plant in Sapporo, Japan, was collected and used to validate our MFQPCR system for multiple viruses. High-throughput quantitative information was obtained with a quantification limit of 2 copies/μl of cDNA/DNA. Using this MFQPCR system, we could simultaneously quantify multiple viral pathogens in environmental water samples. The viral quantities obtained using MFQPCR were similar to those determined by conventional quantitative PCR. Thus, the MFQPCR system developed in this study can provide direct and quantitative information for viral pathogens, which is essential for risk assessments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.