ArticleThe journal of headache and pain2014
Expression of 5-HT3 receptors and TTX resistant sodium channels (Na(V)1.8) on muscle nerve fibers in pain-free humans and patients with chronic myofascial temporomandibular disorders.
Article in The journal of headache and pain, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05720065 (Peripheral TMD Pain Mechanisms and the Effect by Botulinum Toxin A. A Randomized, Controlled, Double-blind Study), which is not on this map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Peripheral TMD Pain Mechanisms and the Effect by Botulinum Toxin A. A Randomized, Controlled, Double-blind Study
Who cites it
31 citing papers in PubMed, 37 citations in OpenAlex.
- Repeated buffered acidic saline infusion in the human masseter muscle as a putative experimental pain model.Scientific reports · 2019Trial
- Repeated tender point injections of granisetron alleviate chronic myofascial pain--a randomized, controlled, double-blinded trial.The journal of headache and pain · 2015Trial
- CRISPR Epigenome Editing of IL1R1 Expression of Neurons that Innervate the Temporomandibular Joint or Knee Causes Reduced Sensitization to Osteoarthritic Environment.Annals of biomedical engineering · 2026Article
- Combination of High-Fat Diet and Chronic Unpredictable Stress Synergistically Induces Osteoarthritis-Like Changes in Temporomandibular Joints in Rats.Mediators of inflammation · 2026Article
- Nav1.8 and Chronic Pain: From Laboratory Animals to Clinical Patients.Biomolecules · 2025Review
- Pharmacologically enabling the degradation of Na V 1.8 channels to reduce neuropathic pain.Pain · 2025Article
- Temporomandibular disorders and mental health: shared etiologies and treatment approaches.The journal of headache and pain · 2025Review
- The Role of the Insular Cortex and Serotonergic System in the Modulation of Long-Lasting Nociception.Cells · 2024Article
- Psoriatic Arthritis Involving TMJ: A Review on Pathogenesis and Consideration on Eventual Gender Differences.Dentistry journal · 2024Review
- Targeted Therapy for Orofacial Pain: A Novel Perspective for Precision Medicine.Journal of personalized medicine · 2023Review
- Disrupted Spontaneous Neural Activity and Its Interaction With Pain and Emotion in Temporomandibular Disorders.Frontiers in neuroscience · 2022Article
- Preclinical models of deep craniofacial nociception and temporomandibular disorder pain.The Japanese dental science review · 2021Review
- Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles.Scientific reports · 2021Article
- Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants.Scientific reports · 2021Article
- Association between convergence insufficiency and temporomandibular disorder cross-sectional study.Clinical oral investigations · 2021Article
- Density of nerve fibres and expression of substance P, NR2B-receptors and nerve growth factor in healthy human masseter muscle: An immunohistochemical study.Journal of oral rehabilitation · 2021Article
- A Female Psoriatic Arthritis Patient Involving the TMJ.Case reports in dentistry · 2021Article
- Polymorphisms in the HTR2A and HTR3A Genes Contribute to Pain in TMD Myalgia.Frontiers in oral health · 2021Article
- EXPRESSION OF SEROTONIN RECEPTOR SUBTYPE 3A (5HT3A) ON RAT TRIGEMINAL SENSORY NEURONS.Texas journal of microscopy · 2021Article
- Contribution of central sensitization to stress-induced spreading hyperalgesia in rats with orofacial inflammation.Molecular brain · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious studies have shown that 5-HT3-antagonists reduce muscle pain, but there are no studies that have investigated the expression of 5-HT3-receptors in human muscles. Also, tetrodotoxin resistant voltage gated sodium-channels (NaV) are involved in peripheral sensitization and found in trigeminal ganglion neurons innervating the rat masseter muscle. This study aimed to investigate the frequency of nerve fibers that express 5-HT3A-receptors alone and in combination with NaV1.8 sodium-channels in human muscles and to compare it between healthy pain-free men and women, the pain-free masseter and tibialis anterior muscles, and patients with myofascial temporomandibular disorders (TMD) and pain-free controls.
methodsThree microbiopsies were obtained from the most bulky part of the tibialis and masseter muscles of seven and six healthy men and seven and six age-matched healthy women, respectively, while traditional open biopsies were obtained from the most painful spot of the masseter of five female patients and from a similar region of the masseter muscle of five healthy, age-matched women. The biopsies were processed by routine immunohistochemical methods. The biopsy sections were incubated with monoclonal antibodies against the specific axonal marker PGP 9.5, and polyclonal antibodies against the 5-HT3A-receptors and NaV1.8 sodium-channels.
resultsA similar percentage of nerve fibers in the healthy masseter (85.2%) and tibialis (88.7%) muscles expressed 5-HT3A-receptors. The expression of NaV1.8 by 5-HT3A positive nerve fibers associated with connective tissue was significantly higher than nerve fibers associated with myocytes (P < .001). In the patients, significantly more fibers per section were found with an average of 3.8 ± 3 fibers per section in the masseter muscle compared to 2.7 ± 0.2 in the healthy controls (P = .024). Further, the frequency of nerve fibers that co-expressed NaV1.8 and 5-HT3A receptors was significantly higher in patients (42.6%) compared to healthy controls (12.0%) (P < .001).
conclusionsThis study showed that the 5-HT3A-receptor is highly expressed in human masseter and tibialis muscles and that there are more nerve fibers that express 5-HT3A-receptors in the masseter of women with myofascial TMD compared to healthy women. These findings indicate that 5-HT3-receptors might be up-regulated in myofascial TMD and could serve as potential biomarkers of chronic muscle pain.
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Registered trials
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