ArticleBiology open2014
The high molecular weight neurofilament subunit plays an essential role in axonal outgrowth and stabilization.
Article in Biology open, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Charcot-Marie-Tooth disease type 2CC due toJournal of neurology, neurosurgery, and psychiatry · 2022Observational
- ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation.Experimental neurobiology · 2020Article
- Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity.Molecular biology of the cell · 2019Article
- Neurofilament heavy polypeptide protects against reduction in synaptopodin expression and prevents podocyte detachment.Scientific reports · 2018Article
- GM1 ganglioside prevents axonal regeneration inhibition and cognitive deficits in a mouse model of traumatic brain injury.Scientific reports · 2018Article
- Counteracting roles of MHCI and CD8Molecular neurodegeneration · 2018Article
- Assembly and turnover of neurofilaments in growing axonal neurites.Biology open · 2018Article
- Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics.Biology open · 2017Article
- Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury.Scientific reports · 2017Article
- MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway.Frontiers in cellular neuroscience · 2017Article
- Exacerbation of Charcot-Marie-Tooth type 2E neuropathy following traumatic nerve injury.Brain research · 2015Article
- Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.Stem cells international · 2015Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurofilaments (NFs) are thought to provide structural support to mature axons via crosslinking of cytoskeletal elements mediated by the C-terminal region of the high molecular weight NF subunit (NF-H). Herein, we inhibited NF-H expression in differentiating mouse NB2a/d1 cells with shRNA directed against murine NF-H without affecting other NF subunits, microtubules or actin. shRNA-mediated NF-H knockdown not only in compromised of late-stage axonal neurite stabilization but also compromised early stages of axonal neurite elongation. Expression of exogenous rat NF-H was able to compensate for knockdown of endogenous NF-H and restored the development and stabilization of axonal neurites. This rescue was prevented by simultaneous treatment with shRNA that inhibited both rat and murine NF-H, or by expression of exogenous rat NF-H lacking the C-terminal sidearm during knockdown of endogenous NF-H. Demonstration of a role for NF-H in the early stages of axonal elaboration suggests that axonal stabilization is not delayed until synaptogenesis, but rather that the developing axon undergoes sequential NF-H-mediated stabilization along its length in a proximal-distal manner, which supports continued pathfinding in distal, unstabilized regions.
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Registered trials
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