ArticlePloS one2014
Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Investigation of the utility of the 1.1B4 cell as a model human beta cell line for study of persistent enteroviral infection.Scientific reports · 2021Article
- The supply chain of human pancreatic β cell lines.The Journal of clinical investigation · 2019Review
- New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines.BMC biotechnology · 2019Article
- The oncogene KRAS promotes cancer cell dissemination by stabilizing spheroid formation via the MEK pathway.BMC cancer · 2018Article
- Virus-like infection induces human β cell dedifferentiation.JCI insight · 2018Article
- β-Cell glutamate signaling: Its role in incretin-induced insulin secretion.Journal of diabetes investigation · 2016Review
- Xenotropic retrovirus Bxv1 in human pancreatic β cell lines.The Journal of clinical investigation · 2016Article
- Early and Late G1/S Cyclins and Cdks Act Complementarily to Enhance Authentic Human β-Cell Proliferation and Expansion.Diabetes · 2015Article
- FUCCI sensors: powerful new tools for analysis of cell proliferation.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of cell cycle in beta cells is poorly understood, especially in humans. We exploited here the recently described human pancreatic beta cell line EndoC-βH2 to set up experimental systems for cell cycle studies. We derived 2 populations from EndoC-βH2 cells that stably harbor the 2 genes encoding the Fucci fluorescent indicators of cell cycle, either from two vectors, or from a unique bicistronic vector. In proliferating non-synchronized cells, the 2 Fucci indicators revealed cells in the expected phases of cell cycle, with orange and green cells being in G1 and S/G2/M cells, respectively, and allowed the sorting of cells in different substeps of G1. The Fucci indicators also faithfully red out alterations in human beta cell proliferative activity since a mitogen-rich medium decreased the proportion of orange cells and inflated the green population, while reciprocal changes were observed when cells were induced to cease proliferation and increased expression of some beta cell genes. In the last situation, acquisition of a more differentiated beta cell phenotype correlates with an increased intensity in orange fluorescence. Hence Fucci beta cell lines provide new tools to address important questions regarding human beta cell cycle and differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.