ArticleProceedings of the National Academy of Sciences of the United States of America2014
Caenorhabditis elegans RSD-2 and RSD-6 promote germ cell immortality by maintaining small interfering RNA populations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 55 citations in OpenAlex.
- Characterizing the role of RSD-6 in the biogenesis of virus-derived small interfering RNAs and the modulation of viral pathogenesis.Journal of virology · 2026Article
- NRDE2 Interacts with an Early Transcription Elongation Complex and Widely Impacts Gene Expression.International journal of molecular sciences · 2025Article
- Characterizing the role of RSD-6 in the biogenesis of virus-derived small interfering RNAs and the modulation of viral pathogenesis.bioRxiv : the preprint server for biology · 2025Article
- Article
- Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activity.bioRxiv : the preprint server for biology · 2025Article
- ThemicroPublication biology · 2024Article
- Caenorhabditis elegans germ granules are present in distinct configurations and assemble in a hierarchical manner.Development (Cambridge, England) · 2023Article
- Genome-wide association and environmental suppression of the mortal germline phenotype of wild C. elegans.EMBO reports · 2023Article
- Article
- Mutation, selection, and the prevalence of the Caenorhabditis elegans heat-sensitive mortal germline phenotype.G3 (Bethesda, Md.) · 2022Article
- Review
- Transgenerational inheritance of sexual attractiveness via small RNAs enhances evolvability in C. elegans.Developmental cell · 2022Article
- Review
- Article
- Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility.Nucleic acids research · 2021Article
- Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility.Nature communications · 2021Article
- ZSP-1 is a Z granule surface protein required for Z granule fluidity and germline immortality in Caenorhabditis elegans.The EMBO journal · 2021Article
- RNAi pathways repress reprogramming of C. elegans germ cells during heat stress.Nucleic acids research · 2020Article
- Article
- Multigenerational Regulation of theAnnual review of genetics · 2019Review
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Authors and funding
13 authors at 5 institutions in 4 countries.
Funding
Abstract
Germ cells are maintained in a pristine non-aging state as they proliferate over generations. Here, we show that a novel function of the Caenorhabditis elegans RNA interference proteins RNAi spreading defective (RSD)-2 and RSD-6 is to promote germ cell immortality at high temperature. rsd mutants cultured at high temperatures became progressively sterile and displayed loss of small interfering RNAs (siRNAs) that target spermatogenesis genes, simple repeats, and transposons. Desilencing of spermatogenesis genes occurred in late-generation rsd mutants, although defective spermatogenesis was insufficient to explain the majority of sterility. Increased expression of repetitive loci occurred in both germ and somatic cells of late-generation rsd mutant adults, suggesting that desilencing of many heterochromatic segments of the genome contributes to sterility. Nuclear RNAi defective (NRDE)-2 promotes nuclear silencing in response to exogenous double-stranded RNA, and our data imply that RSD-2, RSD-6, and NRDE-2 function in a common transgenerational nuclear silencing pathway that responds to endogenous siRNAs. We propose that RSD-2 and RSD-6 promote germ cell immortality at stressful temperatures by maintaining transgenerational epigenetic inheritance of endogenous siRNA populations that promote genome silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.