ArticleScience (New York, N.Y.)2014
Transcriptional diversity during lineage commitment of human blood progenitors.
Open access · greenAbstract read
In one paragraph
Article in Science (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 187 papers, 1 of them a synthesis that pooled it.
0numbers the graph read from it
0cells of the map it votes in
187citing papers in PubMed, 1 pooled it
16.1field-weighted citation impact, top 1% of its field
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
187 citing papers in PubMed, 1 synthesis or guideline pooled it, 310 citations in OpenAlex.
- Large-Scale Exome-wide Association Analysis Identifies Loci for White Blood Cell Traits and Pleiotropy with Immune-Mediated Diseases.American journal of human genetics · 2016Pooled it
- Different subsets of haematopoietic cells and immune cells in bone marrow between young and older donors.Clinical and experimental immunology · 2021Trial
- Meis1 isoform diversity orchestrates neural progenitor differentiation by regulating ATOH1 degradation at distinct subcellular compartments.PLoS biology · 2026Article
- GFI1B mutations define an emerging form of inherited thrombocytopenia: insights from a case report and literature review.Annals of hematology · 2026Review
- Molecular diagnosis of inherited platelet disorder via a targeted whole-exome virtual gene panel: a 5-year institutional experience.Research and practice in thrombosis and haemostasis · 2026Article
- The functional landscape of alternative splicing in hematopoietic lineage commitment.Nature communications · 2026Article
- Targeting splicing-derived neoantigens for precision cancer immunotherapy.Frontiers in immunology · 2026Review
- Interior Decorative VOCs Elevate T Cell-Mediated Obstructive Lung Disease Risks via Osteogenesis-Driven Lymphoid-Biased Hematopoiesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- An inflammatory T-cell-stromal axis contributes to hematopoietic stem/progenitor cell failure and clonal evolution in human myelodysplastic syndrome.Nature communications · 2025Article
- A Novel EYA1 Splicing Mutation in a Chinese Family With Branchio-Oto Syndrome: A Functional Analysis and Reproductive Intervention.Clinical and experimental otorhinolaryngology · 2025Article
- In-utero exposure to maternal diabetes and DNA methylation alterations in the Next Generation birth cohort.Clinical epigenetics · 2025Article
- Hematopoietic Stem Cell Hierarchies as Novel Biomarkers of Drug Response in Myelodysplastic Syndromes and Acute Myeloid Leukemia.Clinical cancer research : an official journal of the American Association for Cancer Research · 2025Review
- Imbalanced TGFβ signalling and autophagy drive erythroid priming of hematopoietic stem cells in β-thalassemia.Nature communications · 2025Article
- Haematometabolism rewiring in atherosclerotic cardiovascular disease.Nature reviews. Cardiology · 2025Review
- Splicing regulatory dynamics for precision analysis and treatment of heterogeneous leukemias.Science translational medicine · 2025Article
- Machine learning-based prediction reveals kinase MAP4K4 regulates neutrophil differentiation through phosphorylating apoptosis-related proteins.PLoS computational biology · 2025Article
- Formation of tight junction-like structures of zonula occludens 2 in platelet-platelet interaction.Research and practice in thrombosis and haemostasis · 2025Article
- Multi-targeted, NOT gated CAR-T cells as a strategy to protect normal lineages for blood cancer therapy.Frontiers in immunology · 2025Article
- Roles of the NFI/CTF gene family in transcription and development: update 2025.Academia biology · 2025Article
- Nuclear Factor I Family Members are Key Transcription Factors Regulating Gene Expression.Molecular & cellular proteomics : MCP · 2025Article
127 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
67 authors at 12 institutions in 3 countries.
Lu Chen *Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Myrto Kostadima *Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Joost H A Martens *Department of Molecular Biology, Radboud University, Nijmegen, the Netherlands.
Giovanni CanuDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Sara P GarciaDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Ernest TurroDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Kate DownesDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Iain C MacaulaySanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.
Ewa Bielczyk-MaczynskaDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Sophia CoeDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Samantha FarrowDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Pawan PoudelDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Frances BurdenDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Sjoert B G JansenDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
William J AstleDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Antony AttwoodDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Tadbir BarianaDepartment of Haematology, University College London Cancer Institute, London, United Kingdom.
Bernard de BonoCHIME Institute, University College London, Archway Campus, London, United Kingdom.
Alessandra BreschiCentre for Genomic Regulation and University Pompeu Fabra, Barcelona, Spain.
John C ChambersImperial College Healthcare NHS Trust, DuCane Road, London, United Kingdom.
Bridge ConsortiumWellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Fizzah A ChoudryDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Laura ClarkeEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Paul CouplandWellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Martijn van der EntDepartment of Molecular Biology, Radboud University, Nijmegen, the Netherlands.
Wendy N ErberPathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia, Australia.
Joop H JansenDepartment of Laboratory Medicine, Laboratory of Hematology, Radboud University Medical Center, Nijmegen, the Netherlands.
Rémi FavierAssistance Publique-Hopitaux de Paris, Institut National de la Santé et de la Recherche Médicale U1009, Villejuif, France.
Matthew E FenechBiomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich, United Kingdom.
Nicola FoadDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Kathleen FresonCenter for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium.
Chris van GeetCenter for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium.
Keith GomezThe Katharine Dormandy Haemophilia Centre and Thrombosis Unit, Royal Free NHS Trust, London, United Kingdom.
Roderic GuigoCentre for Genomic Regulation and University Pompeu Fabra, Barcelona, Spain.
Daniel HampshireDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Anne M KellyDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Hindrik H D KerstensDepartment of Molecular Biology, Radboud University, Nijmegen, the Netherlands.
Jaspal S KoonerImperial College Healthcare NHS Trust, DuCane Road, London, United Kingdom.
Michael LaffanDepartment of Haematology, Hammersmith Campus, Imperial College Academic Health Sciences Centre, Imperial College London, London, United Kingdom.
Claire LentaigneDepartment of Haematology, Hammersmith Campus, Imperial College Academic Health Sciences Centre, Imperial College London, London, United Kingdom.
Charlotte LabaletteDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Tiphaine MartinDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Stuart MeachamDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Andrew MumfordSchool of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom.
Sylvia NürnbergDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Emilio PalumboCentre for Genomic Regulation and University Pompeu Fabra, Barcelona, Spain.
Bert A van der ReijdenDepartment of Laboratory Medicine, Laboratory of Hematology, Radboud University Medical Center, Nijmegen, the Netherlands.
David RichardsonEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Stephen J SammutDepartment of Oncology, Addenbrooke's Cambridge University Hospital NHS Trust, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Greg SlodkowiczEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Asif U TamuriEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Louella VasquezNHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Katrin VossDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Stephen WattNHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Sarah WestburySchool of Clinical Sciences, University of Bristol, United Kingdom.
Paul FlicekEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Remco LoosEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Nick GoldmanEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Paul BertoneEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Randy J ReadDepartment of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Sylvia RichardsonMedical Research Council Biostatistics Unit, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Ana CvejicDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Nicole Soranzo *Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Willem H Ouwehand *Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Hendrik G Stunnenberg *Department of Molecular Biology, Radboud University, Nijmegen, the Netherlands.
Mattia Frontini *Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
Augusto Rendon *Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.
National Health Service · GBUniversity of Cambridge · GBNHS Blood and Transplant · GBWellcome Trust · GBEuropean Bioinformatics Institute · GBWellcome Sanger Institute · GBKU Leuven · BEUniversity of Bristol · GBLondon Cancer · GBMRC Biostatistics Unit · GBUniversity of Auckland · NZUniversity of East Anglia · GB
Funding
British Heart Foundation FS/12/27/29405British Heart Foundation RG/09/012/28096British Heart Foundation RG/09/12/28096British Heart Foundation RP-PG-0310-1002Cancer Research UK 14953Cancer Research UK C45041/A14953Department of Health RP-PG-0310-1002Medical Research Council MC_UP_0801/1Medical Research Council MR/J011711/1Medical Research Council MR/K006584/1Medical Research Council MR/K023489/1Wellcome Trust 082961Wellcome Trust 082961/Z/07/ZWellcome Trust 084183/Z/07/ZWellcome Trust 095908Wellcome Trust 100140Wellcome Trust WT091310Wellcome Trust WT098051
Abstract
Blood cells derive from hematopoietic stem cells through stepwise fating events. To characterize gene expression programs driving lineage choice, we sequenced RNA from eight primary human hematopoietic progenitor populations representing the major myeloid commitment stages and the main lymphoid stage. We identified extensive cell type-specific expression changes: 6711 genes and 10,724 transcripts, enriched in non-protein-coding elements at early stages of differentiation. In addition, we found 7881 novel splice junctions and 2301 differentially used alternative splicing events, enriched in genes involved in regulatory processes. We demonstrated experimentally cell-specific isoform usage, identifying nuclear factor I/B (NFIB) as a regulator of megakaryocyte maturation-the platelet precursor. Our data highlight the complexity of fating events in closely related progenitor populations, the understanding of which is essential for the advancement of transplantation and regenerative medicine.
Indexed as
Alternative SplicingCell LineageGenetic VariationHematopoiesisHematopoietic Stem CellsHumansNFI Transcription FactorsRNA-Binding ProteinsThrombopoiesisTranscriptomeNFIB protein, humanNFI Transcription FactorsRNA-Binding Proteins
Identifiers
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.