ArticleInvestigative ophthalmology & visual science2014
Astrocyte processes label for filamentous actin and reorient early within the optic nerve head in a rat glaucoma model.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 68 citations in OpenAlex.
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- Retinal Neurodegeneration in an Intraocular Pressure Fluctuation Rat Model.International journal of molecular sciences · 2024Article
- IOP and glaucoma damage: The essential role of optic nerve head and retinal mechanosensors.Progress in retinal and eye research · 2024Review
- Astrocytes in the Optic Nerve Are Heterogeneous in Their Reactivity to Glaucomatous Injury.Cells · 2023Article
- Microglia depletion exacerbates retinal ganglion cell loss in a mouse model of glaucoma.Experimental eye research · 2022Article
- Astrocyte polarization in glaucoma: a new opportunity.Neural regeneration research · 2022Review
- Morphological Changes of Glial Lamina Cribrosa of Rats Suffering from Chronic High Intraocular Pressure.Bioengineering (Basel, Switzerland) · 2022Article
- Quantitative Microstructural Analysis of Cellular and Tissue Remodeling in Human Glaucoma Optic Nerve Head.Investigative ophthalmology & visual science · 2022Article
- Relative Contributions of Intraocular and Cerebrospinal Fluid Pressures to the Biomechanics of the Lamina Cribrosa and Laminar Neural Tissues.Investigative ophthalmology & visual science · 2022Article
- Optic Nerve Head Myelin-Related Protein, GFAP, and Iba1 Alterations in Non-Human Primates With Early to Moderate Experimental Glaucoma.Investigative ophthalmology & visual science · 2022Article
- Mechanobiological responses of astrocytes in optic nerve head due to biaxial stretch.BMC ophthalmology · 2022Article
- Evidence of an Annexin A4 mediated plasma membrane repair response to biomechanical strain associated with glaucoma pathogenesis.Journal of cellular physiology · 2022Article
- Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine if astrocyte processes label for actin and to quantify the orientation of astrocytic processes within the optic nerve head (ONH) in a rat glaucoma model.
methodsChronic intraocular pressure (IOP) elevation was produced by episcleral hypertonic saline injection and tissues were collected after 5 weeks. For comparison, eyes with optic nerve transection were collected at 2 weeks. Fellow eyes served as controls. Axonal degeneration in retrobulbar optic nerves was graded on a scale of 1 to 5. Optic nerve head sections (n ≥ 4 eyes per group) were colabeled with phalloidin (actin marker) and antibodies to astrocytic glial fibrillary acidic protein and aquaporin 4, or axonal tubulin βIII. Confocal microscopy and FIJI software were used to quantify the orientation of actin bundles.
resultsControl ONHs showed stereotypically arranged actin bundles within astrocyte processes. Optic nerve head actin bundle orientation was nearly perpendicular to axons (82.9° ± 6.3° relative to axonal axis), unlike the retrobulbar optic nerve (45.4° ± 28.7°, P < 0.05). With IOP elevation, ONH actin bundle orientation became less perpendicular to axons, even in eyes with no perceivable axonal injury (i.e., 38.8° ± 15.1° in grade 1, P < 0.05 in comparison to control ONHs). With severe injury, ONH actin bundle orientation became more parallel to the axonal axis (24.1° ± 28.4°, P < 0.05 in comparison to control ONHs). Optic nerve head actin bundle orientation in transected optic nerves was unchanged.
conclusionsActin labeling identifies fine astrocyte processes within the ONH. Optic nerve head astrocyte process reorientation occurs early in response to elevated IOP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.