ArticleLab on a chip2014
A multi-channel device for high-density target-selective stimulation and long-term monitoring of cells and subcellular features in C. elegans.
Article in Lab on a chip, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Integrated Microfluidic Platform for Longitudinal Imaging, Functional Assays, and Downstream Proteomics in C. elegans.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Article
- Review
- Microfluidic approach to correlateLab on a chip · 2024Article
- Article
- Article
- Fast, efficient, and accurate neuro-imaging denoising via supervised deep learning.Nature communications · 2022Article
- An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in theMicromachines · 2021Article
- Microfluidic-based imaging of complete Caenorhabditis elegans larval development.Development (Cambridge, England) · 2021Article
- Graphical-model framework for automated annotation of cell identities in dense cellular images.eLife · 2021Article
- High-Temporal-Resolution smFISH Method for Gene Expression Studies inAnalytical chemistry · 2021Article
- Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies.Lab on a chip · 2020Article
- Microfluidic approaches forAnimal cells and systems · 2020Review
- A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans.PLoS genetics · 2020Article
- smFISH in chips: a microfluidic-based pipeline to quantify in situ gene expression in whole organisms.Lab on a chip · 2020Article
- Automated phenotyping and lifespan assessment of aTranslational medicine of aging · 2020Article
- Microfluidic Technologies for High Throughput Screening Through Sorting and On-Chip Culture ofMolecules (Basel, Switzerland) · 2019Review
- Rapid and multi-cycle smFISH enabled by microfluidic ion concentration polarization forBiomicrofluidics · 2019Article
- Digging deeper: methodologies for high-content phenotyping in Caenorhabditis elegans.Lab animal · 2019Review
- Multimodal imaging and high-throughput image-processing for drug screening on living organisms on-chip.Journal of biomedical optics · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Selective cell ablation can be used to identify neuronal functions in multicellular model organisms such as Caenorhabditis elegans. The optogenetic tool KillerRed facilitates selective ablation by enabling light-activated damage of cell or subcellular components in a temporally and spatially precise manner. However, the use of KillerRed requires stimulating (5 min-1 h), culturing (~24 h) and imaging (often repeatedly) a large number of individual animals. Current manual manipulation methods are limited by their time-consuming, labor-intensive nature, and their usage of anesthetics. To facilitate large-scale selective ablation, culturing, and repetitive imaging, we developed a densely-packed multi-channel device and used it to perform high-throughput neuronal ablation on KillerRed-expressing animals. The ability to load worms in identical locations with high loading efficiency allows us to ablate selected neurons in multiple worms simultaneously. Our device also enables continuous observation of animals for 24 h following KillerRed activation, and allows the animals to be recovered for behavioural assays. We expect this multi-channel device to facilitate a broad range of long-term imaging and selective illumination experiments in neuroscience.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.