ArticleDisease models & mechanisms2014
Phenotyping structural abnormalities in mouse embryos using high-resolution episcopic microscopy.
Article in Disease models & mechanisms, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 59 citations in OpenAlex.
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- ZSCAN10 deficiency causes a neurodevelopmental disorder with characteristic oto-facial malformations.Brain : a journal of neurology · 2024Article
- Powerful and accurate detection of temporal gene expression patterns from multi-sample multi-stage single-cell transcriptomics data with TDEseq.Genome biology · 2024Article
- Article
- Quantitative Image Processing for Three-Dimensional Episcopic Images of Biological Structures: Current State and Future Directions.Biomedicines · 2023Review
- Longitudinal in Utero Analysis of Engrailed-1 Knockout Mouse Embryonic Phenotypes Using High-Frequency Ultrasound.Ultrasound in medicine & biology · 2023Article
- PAX Genes in Cardiovascular Development.International journal of molecular sciences · 2022Review
- Inefficient development of syncytiotrophoblasts in theProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The venous system of E14.5 mouse embryos-reference data and examples for diagnosing malformations in embryos with gene deletions.Journal of anatomy · 2022Article
- Detailed characterizations of cranial nerve anatomy in E14.5 mouse embryos/fetuses and their use as reference for diagnosing subtle, but potentially lethal malformations in mutants.Frontiers in cell and developmental biology · 2022Article
- Morphogenesis of the Mammalian Aortic Arch Arteries.Frontiers in cell and developmental biology · 2022Article
- Review
- Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM).Biomedicines · 2021Article
- Msx1 haploinsufficiency modifies the Pax9-deficient cardiovascular phenotype.BMC developmental biology · 2021Article
- High Resolution Episcopic Microscopy for Qualitative and Quantitative Data in Phenotyping Altered Embryos and Adult Mice Using the New "Histo3D" System.Biomedicines · 2021Article
- Hypoglossal Nerve Abnormalities as Biomarkers for Central Nervous System Defects in Mouse Lines Producing Embryonically Lethal Offspring.Frontiers in neuroanatomy · 2021Article
- High-resolution episcopic microscopy enables three-dimensional visualization of plant morphology and development.Plant direct · 2019Article
- TheBiology open · 2019Article
- Standardised imaging pipeline for phenotyping mouse laterality defects and associated heart malformations, at multiple scales and multiple stages.Disease models & mechanisms · 2019Article
- Common and distinct transcriptional signatures of mammalian embryonic lethality.Nature communications · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The arrival of simple and reliable methods for 3D imaging of mouse embryos has opened the possibility of analysing normal and abnormal development in a far more systematic and comprehensive manner than has hitherto been possible. This will not only help to extend our understanding of normal tissue and organ development but, by applying the same approach to embryos from genetically modified mouse lines, such imaging studies could also transform our knowledge of gene function in embryogenesis and the aetiology of developmental disorders. The International Mouse Phenotyping Consortium is coordinating efforts to phenotype single gene knockouts covering the entire mouse genome, including characterising developmental defects for those knockout lines that prove to be embryonic lethal. Here, we present a pilot study of 34 such lines, utilising high-resolution episcopic microscopy (HREM) for comprehensive 2D and 3D imaging of homozygous null embryos and their wild-type littermates. We present a simple phenotyping protocol that has been developed to take advantage of the high-resolution images obtained by HREM and that can be used to score tissue and organ abnormalities in a reliable manner. Using this approach with embryos at embryonic day 14.5, we show the wide range of structural abnormalities that are likely to be detected in such studies and the variability in phenotypes between sibling homozygous null embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.