Trial reportBMC veterinary research2014
A high-concentrate diet induced colonic epithelial barrier disruption is associated with the activating of cell apoptosis in lactating goats.
Trial report in BMC veterinary research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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Who cites it
38 citing papers in PubMed, 74 citations in OpenAlex.
- Feeding a high-grain diet reduces the percentage of LPS clearance and enhances immune gene expression in goat liver.BMC veterinary research · 2015Trial
- Effects of Composite Antimicrobial Peptides on Colonic Microbiota and Barrier Integrity in Holstein Steers.Probiotics and antimicrobial proteins · 2026Article
- Responsive changes of rumen metabolome and fatty acid profiles in blood and milk in dairy cows with different sorting behavior.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Forsythiaside A Ameliorates Inflammation by Regulating the Autophagy in Methotrexate-induced Intestinal Mucositis.Combinatorial chemistry & high throughput screening · 2026Article
- Effects of Soybean Meal Replacement on Growth Performance, Rumen Fermentation, Rumen Microorganisms, and Metabolites in Dumont Lambs.Animals : an open access journal from MDPI · 2025Article
- Enhancing Akkermansia growth via phytohormones: a strategy to modulate the gut-bone axis in postmenopausal osteoporosis therapy.Journal of translational medicine · 2025Article
- Multi-Omics Analysis Reveals the Negative Effects of High-Concentrate Diets on the Colonic Epithelium of Dumont Lambs.Animals : an open access journal from MDPI · 2025Article
- The effects of induced hindgut acidosis in sheep on rumen fermentation and gut permeability.Journal of animal science · 2025Article
- The role ofFrontiers in veterinary science · 2025Article
- Paeoniflorin attenuates sepsis-induced liver injury by reprogramming macrophage polarization via the TLR4/NF-κB pathway.Frontiers in immunology · 2025Article
- Anti-inflammatory effects of bay laurel (Food science & nutrition · 2024Article
- Factors affecting the rumen fluid foaming performance in goat fed high concentrate diet.Frontiers in veterinary science · 2024Article
- Genome wide transcriptome analysis provides bases on hepatic lipid metabolism disorder affected by increased dietary grain ratio in fattening lambs.BMC genomics · 2023Article
- Limosilactobacillus mucosae-derived extracellular vesicles modulates macrophage phenotype and orchestrates gut homeostasis in a diarrheal piglet model.NPJ biofilms and microbiomes · 2023Article
- Effects of a High-Concentrate Diet on the Blood Parameters and Liver Transcriptome of Goats.Animals : an open access journal from MDPI · 2023Article
- Subacute ruminal acidosis in dairy herds: Microbiological and nutritional causes, consequences, and prevention strategies.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Review
- Feedlot diets containing different starch levels and additives change the cecal proteome involved in cattle's energy metabolism and inflammatory response.Scientific reports · 2022Article
- Responsive changes of rumen microbiome and metabolome in dairy cows with different susceptibility to subacute ruminal acidosis.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Article
- Metagenomic insights into the rumen epithelial integrity responses to the vitamin B1 supplement under high-concentrate diets treatments.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn ruminants, lower ruminal pH causes massive disruption of ruminal epithelial structure during periods of feeding high-concentrate diets. However, the influence of excessive organic fatty acids in the lumen of hindgut on the epithelial structure is unclear. In this study, twelve mid-lactating goats were randomly assigned to either a HC diet group (65% concentrate of dry matter; n = 6) or a LC diet group (35% concentrate of dry matter; n = 6) for 10 weeks. The colonic epithelial structure was detected by HE staining and transmission electron microscopy (TEM), and the apoptotic status of epithelial cells was estimated by TUNEL method and caspase activities.
resultsHC goats showed higher level of free lipopolysaccharide (LPS) in rumen fluid (p < 0.01) but not in colonic digesta (p > 0.05), and higher total volatile fatty acid (VFA) concentrations in rumen fluid (p < 0.05) and in colonic digesta (p < 0.01), and higher content of starch in colonic digesta (p < 0.05) compared to LC goats. HC goats demonstrated profound alterations in the colonic epithelial structure and tight junctions (TJ), apparently due to damage of the epithelium with widened TJs space and nuclear breakdown and mitochondrial swelling. HC goats showed higher level of apoptosis in the colonic epithelium with higher proportion of TUNEL-positive apoptotic cells and increases of caspase-3 and -3/7 activities, as well as the lower ratio of bcl-2/bax mRNA expression in the colonic mucosa (p < 0.05). However, β-defense mRNA was significantly down-regulated in the colonic mucosa of HC goats compared to LC (p < 0.05). HC goats showed higher level of TJ proteins including claudin-1 and claudin-4 in the colonic mucosa than LC (p < 0.05). Neither free LPS content in the colonic digesta nor NF-κ B protein expression in tissues showed significant difference between HC and LC goats (p > 0.05).
conclusionsOur results reveal that long-term feeding HC diet to lactating goats causes severe damages to the colonic mucosa barrier associated with activating cells apoptosis.
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