ArticleScientific reports2014
Co-detection and sequencing of genes and transcripts from the same single cells facilitated by a microfluidics platform.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 80 citations in OpenAlex.
- Hybrid BAG-seq: DNA and RNA from the same single nucleus reveals interactions between genomic and transcriptomic landscapes in human tumor samples.Genome biology · 2025Article
- Engineering next-generation microfluidic technologies for single-cell phenomics.Nature genetics · 2025Review
- Advances and applications in single-cell and spatial genomics.Science China. Life sciences · 2025Review
- Single-cell genome and transcriptome sequencing without upfront whole-genome amplification reveals cell state plasticity of melanoma subclones.Nucleic acids research · 2025Article
- HIPSD&R-seq enables scalable genomic copy number and transcriptome profiling.Genome biology · 2024Article
- Single-Cell RNA Sequencing and Combinatorial Approaches for Understanding Heart Biology and Disease.Biology · 2024Review
- Body fluid multiomics in 3PM-guided ischemic stroke management: health risk assessment, targeted protection against health-to-disease transition, and cost-effective personalized approach are envisaged.The EPMA journal · 2024Review
- Beyond single cells: microfluidics empowering multiomics analysis.Analytical and bioanalytical chemistry · 2024Review
- The technological landscape and applications of single-cell multi-omics.Nature reviews. Molecular cell biology · 2023Review
- Methods and applications for single-cell and spatial multi-omics.Nature reviews. Genetics · 2023Review
- Effects of oleic acid and/or exercise on diet-induced thermogenesis and obesity in rats: involvement of beige adipocyte differentiation and macrophage M1 inhibition.Research in pharmaceutical sciences · 2023Article
- Single-Cell Multiomics Techniques: From Conception to Applications.Frontiers in cell and developmental biology · 2022Review
- Single Cell Genomics for Tumor Heterogeneity.Advances in experimental medicine and biology · 2021Article
- Cell Atlas technologies and insights into tissue architecture.The Biochemical journal · 2020Review
- Efficient Production of On-Target Reads for Small RNA Sequencing of Single Cells Using Modified Adapters.Analytical chemistry · 2018Article
- Integrated measurement of intracellular proteins and transcripts in single cells.Lab on a chip · 2018Article
- SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.Genome biology · 2018Article
- Transverse dielectrophoretic-based DNA nanoscale confinement.Scientific reports · 2018Article
- Cell-type-specific brain methylomes profiled via ultralow-input microfluidics.Nature biomedical engineering · 2018Article
- Single Platform for Gene and Protein Expression Analyses Using Luminescent Gold Nanoclusters.ACS omega · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Despite the recent advance of single-cell gene expression analyses, co-measurement of both genomic and transcriptional signatures at the single-cell level has not been realized. However such analysis is necessary in order to accurately delineate how genetic information is transcribed, expressed, and regulated to give rise to an enormously diverse range of cell phenotypes. Here we report on a microfluidics-facilitated approach that allows for controlled separation of cytoplasmic and nuclear contents of a single cell followed by on-chip amplification of genomic DNA and cytoplasmic mRNA. When coupled with off-chip polymerase chain reaction, gel electrophoresis and Sanger sequencing, a panel of genes and transcripts from the same single cell can be co-detected and sequenced. This platform is potentially an enabling tool to permit multiple genomic measurements performed on the same single cells and opens new opportunities to tackle a range of fundamental biology questions including non-genetic cell-to-cell variability, epigenetic regulation, and stem cell fate control. It also helps address clinical challenges such as diagnosing intra-tumor heterogeneity and dissecting complex cellular immune responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.