ArticleThe Journal of cell biology1989
The distribution, abundance and subcellular localization of kinesin.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 179 citations in OpenAlex.
- Heterotrimeric kinesin-2 autoinhibition mediated by interactions of the CC2 and proximal tail domains with the motor domains is essential for cilium formation and maintenance.bioRxiv : the preprint server for biology · 2026Article
- Mitochondrial mechanics nucleates axonal jamming and swelling.bioRxiv : the preprint server for biology · 2026Article
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- Sarcopenia-related changes in serum GLP-1 level affect myogenic differentiation.Journal of cachexia, sarcopenia and muscle · 2024Article
- Microtubule damage shapes the acetylation gradient.Nature communications · 2024Article
- Characterization of the disease-causing mechanism of KIF3B mutations from ciliopathy patients.Frontiers in molecular biosciences · 2024Article
- Molecular architecture of the autoinhibited kinesin-1 lambda particle.Science advances · 2022Article
- Kinesin-1 activity recorded in living cells with a precipitating dye.Nature communications · 2021Article
- Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.Cell death discovery · 2021Article
- A novel labeling strategy reveals that myosin Va and myosin Vb bind the same dendritically polarized vesicle population.Traffic (Copenhagen, Denmark) · 2020Article
- A novel strategy to visualize vesicle-bound kinesins reveals the diversity of kinesin-mediated transport.Traffic (Copenhagen, Denmark) · 2019Article
- Fluorescence microscopy applied to intracellular transport by microtubule motors.Journal of biosciences · 2018Review
- CXCL11 Expression by Keratinocytes Occurs Transiently Between Reaching Confluence and Cellular Compaction.Advances in wound care · 2016Article
- The microtubule-binding protein ensconsin is an essential cofactor of kinesin-1.Current biology : CB · 2013Article
- Recycling of kinesin-1 motors by diffusion after transport.PloS one · 2013Article
- Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport.The Journal of cell biology · 2011Article
- Microtubule-associated protein-like binding of the kinesin-1 tail to microtubules.The Journal of biological chemistry · 2010Article
- The Kinesin-1 tail conformationally restricts the nucleotide pocket.Biophysical journal · 2009Article
- Kinesin-1 structural organization and conformational changes revealed by FRET stoichiometry in live cells.The Journal of cell biology · 2007Article
- Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An antiserum which binds kinesin specifically on Western blots was used to determine the distribution and abundance of chicken kinesin by correlated immunoblotting and immunolocalization. Quantitative immunoblotting showed that the abundance of kinesin varied widely in different cell and tissue types, from 0.039% of total protein in epidermal fibroblasts to 0.309% in sympathetic neurons; of the types examined, only red blood cells lacked detectable kinesin. The molar ratio of tubulin/kinesin varied over a narrower range. To analyze the intracellular distribution of kinesin, cultured fibroblasts were fractionated by sequential extraction with saponin-, Triton X-100-, and SDS-containing buffer. Quantitative blotting of the resulting cell fractions indicated that 68% of fibroblast kinesin is in soluble form, 32% is membrane- or organelle-associated, and none is detectable in cytoskeletal fractions. To visualize this distribution, cells treated by the same extraction protocol were immunofluorescently stained with antikinesin and antitubulin. Without extraction, kinesin staining was located throughout cultured neurons and fibroblasts. However, when fibroblasts were extracted with saponin or Brij 58 before fixation, subsequent staining revealed that the remaining kinesin fraction was colocalized with interphase microtubules, but not with mitotic spindles. Prefixation extraction with Triton abolished antikinesin staining. These data suggest that kinesin may play a role in tubovesicular movement but provide no evidence for a role in mitosis.
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