ArticlePloS one2014
In vivo initiation of clock gene expression rhythmicity in fetal rat suprachiasmatic nuclei.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Fetoplacental Circadian Rhythms Develop and Then Synchronize to the Mother In Utero.Journal of biological rhythms · 2026Article
- Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock.Acta physiologica (Oxford, England) · 2026Article
- Fetoplacental circadian rhythms develop and then synchronize to the motherbioRxiv : the preprint server for biology · 2025Article
- Maternal food-derived signals oscillate in the fetal suprachiasmatic nucleus before its circadian clock develops.PLoS biology · 2025Article
- Circadian ontogenetic metabolomics atlas: an interactive resource with insights from rat plasma, tissues, and feces.Cellular and molecular life sciences : CMLS · 2025Article
- Feeding regime synchronizes circadian clock in choroid plexus - insight into a complex mechanism.Cellular and molecular life sciences : CMLS · 2025Article
- Embryonic Circadian Rhythm Establishment, Homeostasis, and Dysfunction During Organogenesis.Stem cells international · 2025Review
- Review
- Developmental patterning of peptide transcription in the central circadian clock in both sexes.Frontiers in neuroscience · 2023Article
- Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach.PLoS biology · 2022Article
- The circadian phase of antenatal glucocorticoid treatment affects the risk of behavioral disorders.Nature communications · 2020Observational
- Circadian Clock Regulation of Developmental Time in the Kidney.Cell reports · 2020Article
- Circadian Clock, Time-Restricted Feeding and Reproduction.International journal of molecular sciences · 2020Review
- Circuit development in the master clock network of mammals.The European journal of neuroscience · 2020Review
- Challenging the Integrity of Rhythmic Maternal Signals Revealed Gene-Specific Responses in the Fetal Suprachiasmatic Nuclei.Frontiers in neuroscience · 2020Article
- Maternal eating behavior is a major synchronizer of fetal and postnatal peripheral clocks in mice.Cellular and molecular life sciences : CMLS · 2018Article
- Implicit time-place conditioning alters Per2 mRNA expression selectively in striatum without shifting its circadian clocks.Scientific reports · 2018Article
- Ontogeny of Circadian Rhythms and Synchrony in the Suprachiasmatic Nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Constructing the suprachiasmatic nucleus: a watchmaker's perspective on the central clockworks.Frontiers in systems neuroscience · 2015Review
- Embryonic development of circadian clocks in the mammalian suprachiasmatic nuclei.Frontiers in neuroanatomy · 2014Review
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Authors and funding
2 authors.
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Abstract
The mammalian suprachiasmatic nuclei (SCN) and their intrinsic rhythmicity develop gradually during ontogenesis. In the rat, the SCN forms between embryonic day (E) 14 and E17, with gestation terminating at E21-22. Overt SCN rhythmicity is already present in the late embryonic stage. The aim of the present study was to determine when the fetal SCN clock develops in vivo and whether overt rhythmicity results from a functional fetal clock. To achieve this goal, the prenatal development of rhythmic expression of clock genes was measured with a more sensitive method for detection of the clock gene expression than previously. Fetal SCN were collected at 3 h intervals during the 24 h period on E19 and E21 by laser dissection and expression of clock genes (Per2, Nr1d1 and Bmal1) and genes related to cellular activity (c-fos, Avp and Vip) was measured by qRT PCR. At E19, the expression of canonical clock genes Per2 and Bmal1 was not rhythmic; however, the expression of all other studied genes followed clear circadian rhythms. At E21, Per2 and Bmal1 expression exhibited low amplitude but significant rhythmicity. From E19 to E21, the levels of the non-rhythmic transcripts (Per2 and Bmal1) decreased; however, the levels of the rhythmic transcripts (Nr1d1, c-fos, Avp and Vip) increased. In summary, these data demonstrate that at E19, rhythms in Per2 and Bmal1 expression were absent in the fetal SCN; however, the expression of Nr1d1 and other genes related to cellular activity was driven rhythmically. Therefore, at the early stage in vivo, the developing fetal SCN clock could theoretically be entrained by oscillation of Nr1d1 which may be driven by the maternal rather than fetal circadian system.
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