ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2014
Effects of analgesic use on inflammation and hematology in a murine model of venous thrombosis.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
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- New Insights on Tramadol and Immunomodulation.Current oncology reports · 2021Review
- The role of platelets in thrombus fibrosis and vessel wall remodeling after venous thrombosis.Journal of thrombosis and haemostasis : JTH · 2021Article
- Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions.Polymers · 2020Article
- Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2020Article
- A Review of the Effects of Pain and Analgesia on Immune System Function and Inflammation: Relevance for Preclinical Studies.Comparative medicine · 2019Review
- Is thrombotic event a side effect of oral buprenorphine abuse? Two case reports.Clinical case reports · 2019Article
- Analgesic Efficacy and Hematologic Effects of Robenacoxib in Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2018Article
- Effects of buprenorphine in the adrenal, thyroid, and cytokine intra-operative responses in a rat model (Iranian journal of basic medical sciences · 2017Article
- Opioids and Opioid Maintenance Therapies: Their Impact on Monocyte-Mediated HIV Neuropathogenesis.Current HIV research · 2016Review
- Pain and Laboratory Animals: Publication Practices for Better Data Reproducibility and Better Animal Welfare.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Venous thrombosis (VT) is a significant cause of morbidity and mortality in humans. Surgical animal models are crucial in studies investigating the pathogenesis of this disease and evaluating VT therapies. Because inflammation is critical to both the development and resolution of VT, analgesic medications have the potential to adversely affect multiple parameters of interest in VT research. The objective of this study was to determine how several common analgesics affect key variables in a murine ligation model of deep vein thrombosis. Male C57BL/6 mice were randomly assigned to receive either local (bupivacaine) or systemic parenteral analgesia (buprenorphine, tramadol, or carprofen) or 0.9% NaCl (control). All mice underwent laparotomy and ligation of the inferior vena cava, and treatment was continued until euthanasia at 6 or 48 h after surgery. Analysis of harvested tissues and blood included: hematology, thrombus weight, serum and vein-wall cytokines (IL1β, IL6, IL10, TNFα), soluble P-selectin, and vein-wall leukocyte infiltration. Compared with 0.9% NaCl, all of the analgesics affected multiple parameters important to VT research. Carprofen and tramadol affected the most parameters and should not be used in murine models of VT. Although they affected fewer parameters, a single dose of bupivacaine increased thrombus weight at 6 h, and buprenorphine was associated with reduced vein wall macrophages at 48 h. Although we cannot recommend the use of any of the evaluated analgesic dosages in this mouse model of VT, buprenorphine merits additional investigation to ensure the highest level of laboratory animal care and welfare.
Indexed as
Identifiers
25255071PMC4181690W2341310155What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.