ArticleStem cell reports2014
Whole-genome sequencing identifies genetic variances in culture-expanded human mesenchymal stem cells.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Hurdles to overcome for mesenchymal stem cell translation from bench to bedside.World journal of stem cells · 2025Review
- A Pseudotumor in a Mouse Kidney Following Human MSCs Injection.Stem cell reviews and reports · 2023Article
- Article
- Common Postzygotic Mutational Signatures in Healthy Adult Tissues Related to Embryonic Hypoxia.Genomics, proteomics & bioinformatics · 2022Article
- Effects of long-term culture on the biological characteristics and RNA profiles of human bone-marrow-derived mesenchymal stem cells.Molecular therapy. Nucleic acids · 2021Article
- Transplantation of human autologous synovial mesenchymal stem cells with trisomy 7 into the knee joint and 5 years of follow-up.Stem cells translational medicine · 2021Article
- Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation.Stem cells international · 2021Article
- Biosafety evaluation of culture-expanded human chondrocytes with growth factor cocktail: a preclinical study.Scientific reports · 2020Article
- Genetic barcoding reveals clonal dominance in iPSC-derived mesenchymal stromal cells.Stem cell research & therapy · 2020Article
- Molecular Mechanisms Contributing to Mesenchymal Stromal Cell Aging.Biomolecules · 2020Review
- Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency.Nature communications · 2020Article
- A promising iPS-based single-cell cloning strategy revealing signatures of somatic mutations in heterogeneous normal cells.Computational and structural biotechnology journal · 2020Article
- Highly Purified Human Extracellular Vesicles Produced by Stem Cells Alleviate Aging Cellular Phenotypes of Senescent Human Cells.Stem cells (Dayton, Ohio) · 2019Article
- Genetic Stability of Mesenchymal Stromal Cells for Regenerative Medicine Applications: A Fundamental Biosafety Aspect.International journal of molecular sciences · 2019Review
- DSMNC: a database of somatic mutations in normal cells.Nucleic acids research · 2019Article
- Article
- Review
- Major changes of cell function and toxicant sensitivity in cultured cells undergoing mild, quasi-natural genetic drift.Archives of toxicology · 2018Article
- Telomerecat: A ploidy-agnostic method for estimating telomere length from whole genome sequencing data.Scientific reports · 2018Article
- Dopaminergic precursors differentiated from human blood-derived induced neural stem cells improve symptoms of a mouse Parkinson's disease model.Theranostics · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Culture-expanded human mesenchymal stem cells (MSCs) are increasingly used in clinics, yet full characterization of the genomic compositions of these cells is lacking. We present a whole-genome investigation on the genetic dynamics of cultured MSCs under ex vivo establishment (passage 1 [p1]) and serial expansion (p8 and p13). We detected no significant changes in copy-number alterations (CNAs) and low levels of single-nucleotide changes (SNCs) until p8. Strikingly, a significant number (677) of SNCs were found in p13 MSCs. Using a sensitive Droplet Digital PCR assay, we tested the nonsynonymous SNCs detected by whole-genome sequencing and found that they were preexisting low-frequency mutations in uncultured mononuclear cells (∼0.01%) and early-passage MSCs (0.1%-1% at p1 and p8) but reached 17%-36% in p13. Our data demonstrate that human MSCs maintain a stable genomic composition in the early stages of ex vivo culture but are subject to clonal growth upon extended expansion.
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Registered trials
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