Evidence map›Paper›PMID 25254073›Full record

ArticleBiotechnology for biofuels2014

The combination of plant-expressed cellobiohydrolase and low dosages of cellulases for the hydrolysis of sugar cane bagasse.

Mark D Harrison, Zhanying Zhang, Kylie Shand, Barrie Fong Chong, Jason Nichols, Paul Oeller, Ian M O'Hara, William Os Doherty, James L Dale

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Mark D HarrisonSyngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia ; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia.
Zhanying ZhangSyngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia ; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia.
Kylie ShandSyngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia ; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia.
Barrie Fong ChongSyngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia ; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia.
Jason NicholsSyngenta Biotechnology Inc., Research Triangle Park, 3054 East Cornwallis Road, Durham, NC 27709-2257 USA.
Paul OellerSyngenta Biotechnology Inc., Research Triangle Park, 3054 East Cornwallis Road, Durham, NC 27709-2257 USA.
Ian M O'HaraSyngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia ; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia.
William Os DohertyCentre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia.
James L DaleSyngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia ; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George Street, Brisbane, Queensland 4001 Australia.
Queensland University of Technology · AUSyngenta (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe expression of biomass-degrading enzymes (such as cellobiohydrolases) in transgenic plants has the potential to reduce the costs of biomass saccharification by providing a source of enzymes to supplement commercial cellulase mixtures. Cellobiohydrolases are the main enzymes in commercial cellulase mixtures. In the present study, a cellobiohydrolase was expressed in transgenic corn stover leaf and assessed as an additive for two commercial cellulase mixtures for the saccharification of pretreated sugar cane bagasse obtained by different processes.

resultsRecombinant cellobiohydrolase in the senescent leaves of transgenic corn was extracted using a simple buffer with no concentration step. The extract significantly enhanced the performance of Celluclast 1.5 L (a commercial cellulase mixture) by up to fourfold on sugar cane bagasse pretreated at the pilot scale using a dilute sulfuric acid steam explosion process compared to the commercial cellulase mixture on its own. Also, the extracts were able to enhance the performance of Cellic CTec2 (a commercial cellulase mixture) up to fourfold on a range of residues from sugar cane bagasse pretreated at the laboratory (using acidified ethylene carbonate/ethylene glycol, 1-butyl-3-methylimidazolium chloride, and ball-milling) and pilot (dilute sodium hydroxide and glycerol/hydrochloric acid steam explosion) scales. We have demonstrated using tap water as a solvent (under conditions that mimic an industrial process) extraction of about 90% recombinant cellobiohydrolase from senescent, transgenic corn stover leaf that had minimal tissue disruption.

conclusionsThe accumulation of recombinant cellobiohydrolase in senescent, transgenic corn stover leaf is a viable strategy to reduce the saccharification cost associated with the production of fermentable sugars from pretreated biomass. We envisage an industrial-scale process in which transgenic plants provide both fibre and biomass-degrading enzymes for pretreatment and enzymatic hydrolysis, respectively.

Indexed as

BiomassCellobiohydrolaseCellulaseEnzymatic hydrolysisPretreatmentSaccharificationSugar caneTransgenic

Identifiers

PMID25254073
PMCPMC4172943
OpenAlexW2138524894

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.