ArticlePostepy dermatologii i alergologii2014
DAPT in the control of human hair follicle stem cell proliferation and differentiation.
Article in Postepy dermatologii i alergologii, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Expression of nicastrin, NICD1, and Hes1 in NCSTN knockout mice: implications for hidradenitis suppurativa, Alzheimer's, and liver cancer.European journal of medical research · 2024Article
- A systematic summary of survival and death signalling during the life of hair follicle stem cells.Stem cell research & therapy · 2021Review
- Article
- Specific inhibitor of Notch‑3 enhances the sensitivity of NSCLC cells to gemcitabine.Oncology reports · 2018Article
- The use of stem cells in aesthetic dermatology and plastic surgery procedures. A compact review of experimental and clinical applications.Postepy dermatologii i alergologii · 2017Article
- Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathway.Stem cell research & therapy · 2017Article
- Deubiquitylating enzyme, USP9X, regulates proliferation of cells of head and neck cancer lines.Cell proliferation · 2016Article
- Co-regulation of the Notch and Wnt signaling pathways promotes supporting cell proliferation and hair cell regeneration in mouse utricles.Scientific reports · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionStem cells from hair follicle have great therapeutic applications in regenerative medicine as sources of cells for transplantation. The differentiation pathway selected by hair follicle stem cells (HFSC) is largely determined by local microenvironmental signals. In this study, human hair follicle stem cells were treated with Notch signaling blocker to explore a new approach to modulate human hair follicle stem cell proliferation and differentiation in vitro.
aimTo define the functional consequences of blocking the Notch signaling pathway on the proliferation and differentiation of human HSCs. MATERIAL AND
methodsThe human hair follicle stem cells were treated with various concentrations of Notch signaling blocker DAPT (24-diamino-5-phenylthiazole). The viability of the cells was investigated with clonogenicity assays. The expression of stem cell markers, cell cycle and cell apoptosis were analysed by flow cytometry.
resultsNotch blocking leads to promotion of human hair follicle stem cell proliferation and inhibition of differentiation in response to DAPT. The maximum effect of DAPT on the viability of human HFSC was observed at a concentration of 20 µM. We found that DAPT treatment results in downregulation of Hes1 and p21 and upregulation of Wnt10b.
conclusionsγ-Secretase inhibitor DAPT has a modulatory effect on the human HFSC. The DAPT may modulate human hair follicle stem cell proliferation and differentiation through regulation of p21 and Wnt-10b.
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