ArticleMetabolomics : Official journal of the Metabolomic Society2014
A Statistical Analysis of the Effects of Urease Pre-treatment on the Measurement of the Urinary Metabolome by Gas Chromatography-Mass Spectrometry.
Article in Metabolomics : Official journal of the Metabolomic Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Persistent urinary metabolic signatures in children with type 1 diabetes.Next research · 2025Article
- Sample Preparation for Metabolomic Analysis in Exercise Physiology.Biomolecules · 2024Review
- Multi-scale signaling and tumor evolution in high-grade gliomas.Cancer cell · 2024Article
- Optimising a urinary extraction method for non-targeted GC-MS metabolomics.Scientific reports · 2023Article
- Influence of grape consumption on the human microbiome.Scientific reports · 2023Article
- Short-Term Grape Consumption Diminishes UV-Induced Skin Erythema.Antioxidants (Basel, Switzerland) · 2022Article
- Ammonium chloride-induced acidosis exacerbates cystitis and pyelonephritis caused by uropathogenic E. coli.Physiological reports · 2022Article
- Untargeted Metabolomics Study of Three Matrices: Seminal Fluid, Urine, and Serum to Search the Potential Indicators of Prostate Cancer.Frontiers in molecular biosciences · 2022Article
- A resource of lipidomics and metabolomics data from individuals with undiagnosed diseases.Scientific data · 2021Article
- Proteogenomic and metabolomic characterization of human glioblastoma.Cancer cell · 2021Article
- Quantitative NMR-Based Biomedical Metabolomics: Current Status and Applications.Molecules (Basel, Switzerland) · 2020Review
- yippee like 3 (ypel3) is a novel gene required for myelinating and perineurial glia development.PLoS genetics · 2020Article
- A review of strategies for untargeted urinary metabolomic analysis using gas chromatography-mass spectrometry.Metabolomics : Official journal of the Metabolomic Society · 2020Review
- Statistically Driven Metabolite and Lipid Profiling of Patients from the Undiagnosed Diseases Network.Analytical chemistry · 2020Article
- Systematic re-evaluation of the long-used standard protocol of urease-dependent metabolome sample preparation.PloS one · 2020Article
- Automated supervised learning pipeline for non-targeted GC-MS data analysis.Analytica chimica acta: X · 2019Article
- The use of mass spectrometry for analysing metabolite biomarkers in epidemiology: methodological and statistical considerations for application to large numbers of biological samples.European journal of epidemiology · 2016Review
- Silymarin Suppresses Cellular Inflammation By Inducing Reparative Stress Signaling.Journal of natural products · 2015Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Urease pre-treatment of urine has been utilized since the early 1960s to remove high levels of urea from samples prior to further processing and analysis by gas chromatography-mass spectrometry (GC-MS). Aside from the obvious depletion or elimination of urea, the effect, if any, of urease pre-treatment on the urinary metabolome has not been studied in detail. Here, we report the results of three separate but related experiments that were designed to assess possible indirect effects of urease pre-treatment on the urinary metabolome as measured by GC-MS. In total, 235 GC-MS analyses were performed and over 106 identified and 200 unidentified metabolites were quantified across the three experiments. The results showed that data from urease pre-treated samples 1) had the same or lower coefficients of variance among reproducibly detected metabolites, 2) more accurately reflected quantitative differences and the expected ratios among different urine volumes, and 3) increased the number of metabolite identifications. Overall, we observed no negative consequences of urease pre-treatment. In contrast, urease pretreatment enhanced the ability to distinguish between volume-based and biological sample types compared to no treatment. Taken together, these results show that urease pretreatment of urine offers multiple beneficial effects that outweigh any artifacts that may be introduced to the data in urinary metabolomics analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.