ArticlePlant physiology2014
Inducible knockdown of MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE1 reveals roles of galactolipids in organelle differentiation in Arabidopsis cotyledons.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 69 citations in OpenAlex.
- 2-Cys peroxiredoxins and the chaperone cpHSP70 act in concert in chloroplast biogenesis in Arabidopsis seedlings.The Plant cell · 2026Article
- Rice MGD1-Mediated Improvement of Photosynthesis and Crop Yield by Flexible Adaptation to Different Light Conditions.Plant biotechnology journal · 2026Article
- ANTHOCYANIDIN REDUCTASE promotes physical dormancy in Medicago truncatula seeds.Plant physiology · 2025Article
- Chilling or chemical induction of dormancy release in blackcurrant (Ribes nigrum) buds is associated with characteristic shifts in metabolite profiles.The Biochemical journal · 2024Article
- Orchestration of Photosynthesis-Associated Gene Expression and Galactolipid Biosynthesis during Chloroplast Differentiation in Plants.Plant & cell physiology · 2024Article
- OsMGD1-Mediated Membrane Lipid Remodeling Improves Salt Tolerance in Rice.Plants (Basel, Switzerland) · 2024Article
- Exploring the plant lipidome: techniques, challenges, and prospects.Advanced biotechnology · 2024Review
- LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (International journal of molecular sciences · 2023Article
- Drought and heat stress mediated activation of lipid signaling in plants: a critical review.Frontiers in plant science · 2023Review
- Lipid remodeling of contrasting maize (Frontiers in plant science · 2023Article
- Mitochondrial Complex I Disruption Causes Broad Reorchestration of Plant Lipidome Including Chloroplast Lipids.International journal of molecular sciences · 2022Article
- Gene Expression Analysis of Potato (Plants (Basel, Switzerland) · 2022Article
- Plant Acyl-CoA-Binding Proteins-Their Lipid and Protein Interactors in Abiotic and Biotic Stresses.Cells · 2021Review
- The Role of Membranes and Lipid-Protein Interactions in the Mg-Branch of Tetrapyrrole Biosynthesis.Frontiers in plant science · 2021Review
- Chloroplast Lipids Metabolism and Function. A Redox Perspective.Frontiers in plant science · 2021Review
- The roles of chloroplast membrane lipids in abiotic stress responses.Plant signaling & behavior · 2020Article
- Increased ratio of galactolipid MGDG : DGDG induces jasmonic acid overproduction and changes chloroplast shape.The New phytologist · 2020Article
- Dynamic changes in membrane lipid composition of leaves of winter wheat seedlings in response to PEG-induced water stress.BMC plant biology · 2020Article
- Beyond the darkness: recent lessons from etiolation and de-etiolation studies.Journal of experimental botany · 2020Review
- Regulation of Sugar and Storage Oil Metabolism by Phytochrome during De-etiolation.Plant physiology · 2020Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monogalactosyldiacylglycerol (MGDG) is the major lipid constituent of thylakoid membranes and is essential for chloroplast biogenesis in plants. In Arabidopsis (Arabidopsis thaliana), MGDG is predominantly synthesized by inner envelope-localized MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE1 (MGD1); its knockout causes albino seedlings. Because of the lethal phenotype of the null MGD1 mutant, functional details of MGDG synthesis at seedling development have remained elusive. In this study, we used an inducible gene-suppression system to investigate the impact of MGDG synthesis on cotyledon development. We created transgenic Arabidopsis lines that express an artificial microRNA targeting MGD1 (amiR-MGD1) under the control of a dexamethasone-inducible promoter. The induction of amiR-MGD1 resulted in up to 75% suppression of MGD1 expression, although the resulting phenotypes related to chloroplast development were diverse, even within a line. The strong MGD1 suppression by continuous dexamethasone treatment caused substantial decreases in galactolipid content in cotyledons, leading to severe defects in the formation of thylakoid membranes and impaired photosynthetic electron transport. Time-course analyses of the MGD1 suppression during seedling germination revealed that MGDG synthesis at the very early germination stage is particularly important for chloroplast biogenesis. The MGD1 suppression down-regulated genes associated with the photorespiratory pathway in peroxisomes and mitochondria as well as those responsible for photosynthesis in chloroplasts and caused high expression of genes for the glyoxylate cycle. MGD1 function may link galactolipid synthesis with the coordinated transcriptional regulation of chloroplasts and other organelles during cotyledon greening.
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