ArticlePlant physiology2014
Systematic phenotypic screen of Arabidopsis peroxisomal mutants identifies proteins involved in β-oxidation.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Genome-Wide Identification and Characterization of Tomato Fatty Acid β-Oxidase Family GenesInternational journal of molecular sciences · 2024Article
- Identification of Arabidopsis Protein Kinases That Harbor Functional Type 1 Peroxisomal Targeting Signals.Frontiers in cell and developmental biology · 2022Article
- Plasma membrane phylloquinone biosynthesis in nonphotosynthetic parasitic plants.Plant physiology · 2021Article
- E3 ligase, the Oryza sativa salt-induced RING finger protein 4 (OsSIRP4), negatively regulates salt stress responses via degradation of the OsPEX11-1 protein.Plant molecular biology · 2021Article
- Overexpression of AtAGT1 promoted root growth and development during seedling establishment.Plant cell reports · 2019Article
- Plant peroxisomal solute transporter proteins.Journal of integrative plant biology · 2019Review
- The Arabidopsis E3 Ubiquitin Ligase SP1 Targets to Chloroplasts, Peroxisomes, and Mitochondria.Plant physiology · 2018Article
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- Plant peroxisomes: A nitro-oxidative cocktail.Redox biology · 2017Review
- Impact of salt stress, cell death, and autophagy on peroxisomes: quantitative and morphological analyses using small fluorescent probe N-BODIPY.Scientific reports · 2017Article
- Plant peroxisomes: recent discoveries in functional complexity, organelle homeostasis, and morphological dynamics.Current opinion in plant biology · 2016Review
- OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance inFrontiers in plant science · 2016Article
- Peroxisomes sense and respond to environmental cues by regulating ROS and RNS signalling networks.Annals of botany · 2015Review
- Using Co-Expression Analysis and Stress-Based Screens to Uncover Arabidopsis Peroxisomal Proteins Involved in Drought Response.PloS one · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peroxisomes are highly dynamic and multifunctional organelles essential to development. Plant peroxisomes accommodate a multitude of metabolic reactions, many of which are related to the β-oxidation of fatty acids or fatty acid-related metabolites. Recently, several dozens of novel peroxisomal proteins have been identified from Arabidopsis (Arabidopsis thaliana) through in silico and experimental proteomic analyses followed by in vivo protein targeting validations. To determine the functions of these proteins, we interrogated their transfer DNA insertion mutants with a series of physiological, cytological, and biochemical assays to reveal peroxisomal deficiencies. Sugar dependence and 2,4-dichlorophenoxybutyric acid and 12-oxo-phytodienoic acid response assays uncovered statistically significant phenotypes in β-oxidation-related processes in mutants for 20 of 27 genes tested. Additional investigations uncovered a subset of these mutants with abnormal seed germination, accumulation of oil bodies, and delayed degradation of long-chain fatty acids during early seedling development. Mutants for seven genes exhibited deficiencies in multiple assays, strongly suggesting the involvement of their gene products in peroxisomal β-oxidation and initial seedling growth. Proteins identified included isoforms of enzymes related to β-oxidation, such as acyl-CoA thioesterase2, acyl-activating enzyme isoform1, and acyl-activating enzyme isoform5, and proteins with functions previously unknown to be associated with β-oxidation, such as Indigoidine synthase A, Senescence-associated protein/B12D-related protein1, Betaine aldehyde dehydrogenase, and Unknown protein5. This multipronged phenotypic screen allowed us to reveal β-oxidation proteins that have not been discovered by single assay-based mutant screens and enabled the functional dissection of different isoforms of multigene families involved in β-oxidation.
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