ArticleMolecular biology of the cell2014
Membrane tethering by the atlastin GTPase depends on GTP hydrolysis but not on forming the cross-over configuration.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Atlastin-2-mediated endoplasmic reticulum membrane tethering is critical for flavivirus replication.PLoS biology · 2026Article
- Dissecting the mechanism of atlastin-mediated homotypic membrane fusion at the single-molecule level.Nature communications · 2024Article
- Membrane fusion byMolecular biology of the cell · 2022Article
- A role for endoplasmic reticulum dynamics in the cellular distribution of microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Reconstitution of human atlastin fusion activity reveals autoinhibition by the C terminus.The Journal of cell biology · 2022Article
- The hypervariable region of atlastin-1 is a site for intrinsic and extrinsic regulation.The Journal of cell biology · 2021Article
- CAND1 regulates lunapark for the proper tubular network of the endoplasmic reticulum.Scientific reports · 2019Article
- GTP hydrolysis promotes disassembly of the atlastin crossover dimer during ER fusion.The Journal of cell biology · 2018Article
- A hereditary spastic paraplegia-associated atlastin variant exhibits defective allosteric coupling in the catalytic core.The Journal of biological chemistry · 2018Article
- Molecular mechanisms of atlastin-mediated ER membrane fusion revealed by a FRET-based single-vesicle fusion assay.Scientific reports · 2017Article
- Timing and Reset Mechanism of GTP Hydrolysis-Driven Conformational Changes of Atlastin.Structure (London, England : 1993) · 2017Article
- The crossover conformational shift of the GTPase atlastin provides the energy driving ER fusion.The Journal of cell biology · 2017Article
- Article
- Visualization and Sequencing of Membrane Remodeling Leading to Influenza Virus Fusion.Journal of virology · 2016Article
- Invited review: Mechanisms of GTP hydrolysis and conformational transitions in the dynamin superfamily.Biopolymers · 2016Review
- Article
- Physiological lipid composition is vital for homotypic ER membrane fusion mediated by the dynamin-related GTPase Sey1p.Scientific reports · 2016Article
- Structures of the yeast dynamin-like GTPase Sey1p provide insight into homotypic ER fusion.The Journal of cell biology · 2015Article
- SNAREs support atlastin-mediated homotypic ER fusion in Saccharomyces cerevisiae.The Journal of cell biology · 2015Article
- ER network formation and membrane fusion by atlastin1/SPG3A disease variants.Molecular biology of the cell · 2015Article
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4 authors at 2 institutions in 1 country.
Funding
Abstract
The membrane-anchored atlastin GTPase couples nucleotide hydrolysis to the catalysis of homotypic membrane fusion to form a branched endoplasmic reticulum network. Trans dimerization between atlastins anchored in opposing membranes, accompanied by a cross-over conformational change, is thought to draw the membranes together for fusion. Previous studies on the conformational coupling of atlastin to its GTP hydrolysis cycle have been carried out largely on atlastins lacking a membrane anchor. Consequently, whether fusion involves a discrete tethering step and, if so, the potential role of GTP hydrolysis and cross-over in tethering remain unknown. In this study, we used membrane-anchored atlastins in assays that separate tethering from fusion to dissect the requirements for each. We found that tethering depended on GTP hydrolysis, but, unlike fusion, it did not depend on cross-over. Thus GTP hydrolysis initiates stable head-domain contact in trans to tether opposing membranes, whereas cross-over formation plays a more pivotal role in powering the lipid rearrangements for fusion.
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