ArticleMolecular biology of the cell2014
TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- TORC2 coordinates MBF-dependent transcription and restrains oxidative stress responses during DNA replication stress in fission yeast.The Journal of biological chemistry · 2026Article
- TRPM2: a pivotal player in tumor progression and a promising therapeutic target.Cancer cell international · 2025Review
- Article
- mTORC2: a multifaceted regulator of autophagy.Cell communication and signaling : CCS · 2023Review
- Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast.FEMS yeast research · 2023Article
- Article
- A VASt-domain protein regulates autophagy, membrane tension, and sterol homeostasis in rice blast fungus.Autophagy · 2021Article
- Expanding TOR Complex 2 Signaling: Emerging Regulators and New Connections.Frontiers in cell and developmental biology · 2021Review
- Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch9.Molecular & cellular proteomics : MCP · 2020Article
- Chloroplast avoidance movement: a novel paradigm of ROS signalling.Photosynthesis research · 2020Review
- Review
- Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signaling.MicrobiologyOpen · 2019Article
- Cyclodipeptides fromPeerJ · 2019Article
- Phosphorylation by the stress-activated MAPK Slt2 down-regulates the yeast TOR complex 2.Genes & development · 2018Article
- Target of rapamycin complex 2-dependent phosphorylation of the coat protein Pan1 by Akl1 controls endocytosis dynamics inThe Journal of biological chemistry · 2018Article
- Heterologous mammalian Akt disrupts plasma membrane homeostasis by taking over TORC2 signaling in Saccharomyces cerevisiae.Scientific reports · 2018Article
- Guidelines and recommendations on yeast cell death nomenclature.Microbial cell (Graz, Austria) · 2018Review
- A conserved signaling network monitors delivery of sphingolipids to the plasma membrane in budding yeast.Molecular biology of the cell · 2017Article
- The TORC2-Dependent Signaling Network in the Yeast Saccharomyces cerevisiae.Biomolecules · 2017Review
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The evolutionarily conserved mTOR complex 2 (mTORC2) signaling pathway is an important regulator of actin cytoskeletal architecture and, as such, is a candidate target for preventing cancer cell motility and invasion. Remarkably, the precise mechanism(s) by which mTORC2 regulates the actin cytoskeleton have remained elusive. Here we show that in budding yeast, TORC2 and its downstream kinase Ypk1 regulate actin polarization by controlling reactive oxygen species (ROS) accumulation. Specifically, we find that TORC2-Ypk1 regulates actin polarization both by vacuole-related ROS, controlled by the phospholipid flippase kinase Fpk1 and sphingolipids, and by mitochondria-mediated ROS, controlled by the PKA subunit Tpk3. In addition, we find that the protein kinase C (Pkc1)/MAPK cascade, a well-established regulator of actin, acts downstream of Ypk1 to regulate ROS, in part by promoting degradation of the oxidative stress responsive repressor, cyclin C. Furthermore, we show that Ypk1 regulates Pkc1 activity through proper localization of Rom2 at the plasma membrane, which is also dependent on Fpk1 and sphingolipids. Together these findings demonstrate important links between TORC2/Ypk1 signaling, Fpk1, sphingolipids, Pkc1, and ROS as regulators of actin and suggest that ROS may play an important role in mTORC2-dependent dysregulation of the actin cytoskeleton in cancer cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.