Evidence map›Paper›PMID 25253152›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

Examination of galectin localization using confocal microscopy.

Daniel Giuliano Cerri, Connie M Arthur, Lílian Cataldi Rodrigues, Marise Lopes Fermino, Lenaldo Branco Rocha, Sean R Stowell, Marcelo Dias Baruffi

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Exploring the Galectin Network by Light and Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  3. Galectin-9 Is a Novel Regulator of Epithelial Restitution.The American journal of pathology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Daniel Giuliano CerriFaculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical, Toxicological and Bromatological Analysis, University of Sao Paulo, Ribeirão Preto-S, Brazil.
Connie M Arthur
Lílian Cataldi Rodrigues
Marise Lopes Fermino
Lenaldo Branco Rocha
Sean R Stowell
Marcelo Dias Baruffi

Funding

Training Grant in Transfusion Medicine (T32)T32HL069769 · NHLBI · EMORY UNIVERSITY · PI Una T O'Doherty, John D Roback · 2004 to 2026
$2.6M
NHLBI NIH HHS T32 HL069769
6 · The paper itself

Abstract

Confocal microscopy provides a unique modality to examine the expression and localization of biomolecules in a variety of settings. Using this technique, an image is acquired from the focal plane of the objective using focused laser light, making it possible to work within the resolution limit of the optical system. In addition, by acquiring multiple images from a variety of focal planes, stacked series of images can provide clear spatial localization of a probed structure or protein. We describe herein the immunofluorescence methods for galectin staining in frozen sections of tissue for galectin localization using confocal microscopy.

Indexed as

Fluorescent Antibody TechniqueGalectinsGlassMicroscopy, ConfocalProtein TransportGalectins

Identifiers

PMID25253152
PMCPMC5777144

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.