ArticleAmerican journal of physiology. Cell physiology2014
Functional mitochondrial analysis in acute brain sections from adult rats reveals mitochondrial dysfunction in a rat model of migraine.
Article in American journal of physiology. Cell physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 51 citations in OpenAlex.
- Hippocampal Bioenergetics and Metabolic Profiling Identifies Fatty Acid Oxidation as a Potential Therapeutic Target in Traumatic Brain Injury.Molecular neurobiology · 2026Article
- Inhibitory GABAergic Neuron Loss due to Oxidative Damage During Ex Vivo Acute Brain Slice Preparation Influences Genesis and Dynamics of Epileptiform Activity.Journal of neurochemistry · 2026Article
- Oxidative Stress and Mitophagy in Rats With Nitroglycerin/Inflammatory Soup-Induced Migraine: A Comparative Study.Pain research & management · 2026Article
- Associations between dietary intake of mitochondria-related nutrients with the risk of migraine: a prospective study of 202,656 participants.The journal of headache and pain · 2025Article
- Possible Role of Novel Mitochondrial Subsets in Migraine.Life (Basel, Switzerland) · 2025Review
- Assessment of Phase-Dependent Alterations in Cortical Glycolytic and Mitochondrial Metabolism Following Ischemic Stroke.ASN neuro · 2025Article
- Cerebral hypoperfusion exacerbates vascular dysfunction after traumatic brain injury.Experimental neurology · 2024Article
- A semi-automatic method for extracting mitochondrial cristae characteristics from 3D focused ion beam scanning electron microscopy data.Communications biology · 2024Article
- Tracing the path of disruption:CNS neuroscience & therapeutics · 2024Review
- Peroxynitrite Contributes to Behavioral Responses, Increased Trigeminal Excitability, and Changes in Mitochondrial Function in a Preclinical Model of Migraine.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Thioredoxin-1 regulates self-renewal and differentiation of murine hematopoietic stem cells through p53 tumor suppressor.Experimental hematology & oncology · 2022Article
- Alterations in metabolic flux in migraine and the translational relevance.The journal of headache and pain · 2022Review
- Review
- Characterizing region-specific glucose metabolic profile of the rodent brain using Seahorse XFe96 analyzer.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022Article
- Article
- Mild traumatic brain injury increases vulnerability to cerebral ischemia in mice.Experimental neurology · 2021Article
- Characterizing brain metabolic function ex vivo with acute mouse slice punches.STAR protocols · 2021Article
- Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution.Cell reports methods · 2021Article
- Curbing action potential generation or ATP-synthase leads to a decrease in in-cell pyruvate dehydrogenase activity in rat cerebrum slices.Scientific reports · 2021Article
- The Role of Metabolism in Migraine Pathophysiology and Susceptibility.Life (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mitochondrial dysfunction has been implicated in many neurological disorders that only develop or are much more severe in adults, yet no methodology exists that allows for medium-throughput functional mitochondrial analysis of brain sections from adult animals. We developed a technique for quantifying mitochondrial respiration in acutely isolated adult rat brain sections with the Seahorse XF Analyzer. Evaluating a range of conditions made quantifying mitochondrial function from acutely derived adult brain sections from the cortex, cerebellum, and trigeminal nucleus caudalis possible. Optimization of this technique demonstrated that the ideal section size was 1 mm wide. We found that sectioning brains at physiological temperatures was necessary for consistent metabolic analysis of trigeminal nucleus caudalis sections. Oxygen consumption in these sections was highly coupled to ATP synthesis, had robust spare respiratory capacities, and had limited nonmitochondrial respiration, all indicative of healthy tissue. We demonstrate the effectiveness of this technique by identifying a decreased spare respiratory capacity in the trigeminal nucleus caudalis of a rat model of chronic migraine, a neurological disorder that has been associated with mitochondrial dysfunction. This technique allows for 24 acutely isolated sections from multiple brain regions of a single adult rat to be analyzed simultaneously with four sequential drug treatments, greatly advancing the ability to study mitochondrial physiology in adult neurological disorders.
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Registered trials
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