ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2014
Development of pod borer-resistant transgenic chickpea using a pod-specific and a constitutive promoter-driven fused cry1Ab/Ac gene.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exploring Chickpea Germplasm Diversity for Broadening the Genetic Base Utilizing Genomic Resourses.Frontiers in genetics · 2022Pooled it
- Novel codon-optimized cry1Ac34 gene from Indian Bt isolate confers resistance against Helicoverpa armigera in Transgenic tobacco.Archives of microbiology · 2026Article
- Multi-omics reveals DEAD-box RNA helicase 20 as key protein enhancing wilt resistance in transgenic chickpea.Plant cell reports · 2025Article
- Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- The root-specific NtR12 promoter-based expression of RIP increased the resistance against bacterial wilt disease in tobacco.Molecular biology reports · 2022Article
- Chickpea (Plants (Basel, Switzerland) · 2022Review
- Identifying Soybean Pod Borer (International journal of molecular sciences · 2022Article
- Evaluation of transgenic chickpea harboring codon-modified Vip3Aa against gram pod borer (Helicoverpa armigera H.).PloS one · 2022Article
- Genetic transformation of legumes: an update.Plant cell reports · 2021Review
- Symbiotic nitrogen fixation and endophytic bacterial community structure in Bt-transgenic chickpea (Cicer arietinum L).Scientific reports · 2020Article
- An Improved Transformation System for Cowpea (Frontiers in plant science · 2019Article
- Development of a rapid and highly efficientGM crops & food · 2019Article
- An Update on Genetic Modification of Chickpea for Increased Yield and Stress Tolerance.Molecular biotechnology · 2018Review
- Viruses and Phytoparasitic Nematodes ofFrontiers in plant science · 2018Review
- Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice.Scientific reports · 2017Article
- Bt Jute Expressing Fused δ-Endotoxin Cry1Ab/Ac for Resistance toFrontiers in plant science · 2017Article
- Expression of a Chimeric Gene Encoding Insecticidal Crystal Protein Cry1Aabc ofFrontiers in plant science · 2017Article
- Article
- Genome Wide Identification of LIM Genes in Cicer arietinum and Response of Ca-2LIMs in Development, Hormone and Pathogenic Stress.PloS one · 2015Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWe studied pod-specific msg promoter from soybean and developed different transgenic lines of chickpea expressing fused cry1Ab/Ac constitutively and pod specifically for resistance against the destructive pest Helicoverpa armigera. Crystal (Cry) proteins derived from the soil bacterium Bacillus thuringiensis (Bt) play an important role in controlling infestation of Helicoverpa armigera, which has been considered a serious problem in chickpea productivity. This study was undertaken to overcome the problem by introducing fused cry1Ab/Ac insecticidal gene under the control of pod-specific soybean msg promoter as well as rice actin1 promoter into chickpea var. DCP 92-3 by Agrobacterium-mediated transformation. Transgenic chickpea lines were characterized by real-time PCR, ELISA and insect bioassay. Expression of fused cry gene under constitutive and pod-specific promoter results in increase of 77- and 110-fold, respectively, compared to non-transgenic control plants. Levels of Cry toxins produced under the control of actin1 and soybean msg promoter were also estimated by ELISA in the leaves and pods, respectively. The higher expression of fused cry gene caused a lethal effect in larvae. The results of insect bioassay study revealed significant reduction in the survival rate of H. armigera reared on transgenic chickpea twigs as well as on pods. Pod-specific promoter-driven fused cry gene provides better and significant management strategy of pest control of chickpea without phenotypic cost.
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