ArticlePloS one2014
Efficient illumination for microsecond tracking microscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- Leveraging Partial Coherence to Enhance Nanoparticle Detection Sensitivity and Throughput in Interferometric Scattering Microscopy.ACS photonics · 2025Article
- Spatiotemporal dynamics of the proton motive force on single bacterial cells.Science advances · 2024Article
- Dynamic stiffening of the flagellar hook.Nature communications · 2022Article
- Temperature controlled high-throughput magnetic tweezers show striking difference in activation energies of replicating viral RNA-dependent RNA polymerases.Nucleic acids research · 2020Article
- High spatiotemporal resolution data from a custom magnetic tweezers instrument.Data in brief · 2020Article
- High-Performance Image-Based Measurements of Biological Forces and Interactions in a Dual Optical Trap.ACS nano · 2018Article
- Correction-free force calibration for magnetic tweezers experiments.Scientific reports · 2018Article
- Holographic Traction Force Microscopy.Scientific reports · 2018Article
- Catch bond drives stator mechanosensitivity in the bacterial flagellar motor.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Impact of fluorescent protein fusions on the bacterial flagellar motor.Scientific reports · 2017Article
- Reduction of coherent artefacts in super-resolution fluorescence localisation microscopy.Journal of microscopy · 2016Article
- Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging.Nature protocols · 2016Article
- Interferometric Scattering Microscopy for the Study of Molecular Motors.Methods in enzymology · 2016Article
- High Spatiotemporal-Resolution Magnetic Tweezers: Calibration and Applications for DNA Dynamics.Biophysical journal · 2015Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The possibility to observe microsecond dynamics at the sub-micron scale, opened by recent technological advances in fast camera sensors, will affect many biophysical studies based on particle tracking in optical microscopy. A main limiting factor for further development of fast video microscopy remains the illumination of the sample, which must deliver sufficient light to the camera to allow microsecond exposure times. Here we systematically compare the main illumination systems employed in holographic tracking microscopy, and we show that a superluminescent diode and a modulated laser diode perform the best in terms of image quality and acquisition speed, respectively. In particular, we show that the simple and inexpensive laser illumination enables less than 1 μs camera exposure time at high magnification on a large field of view without coherence image artifacts, together with a good hologram quality that allows nm-tracking of microscopic beads to be performed. This comparison of sources can guide in choosing the most efficient illumination system with respect to the specific application.
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