ArticleJournal of applied microbiology2014
The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage.
Article in Journal of applied microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Persistence of SARS-CoV-2 and its surrogate, bacteriophage Phi6, on surfaces and in water.Applied and environmental microbiology · 2023Article
- Lyophilization to enable distribution of ChAdOx1 and ChAdOx2 adenovirus-vectored vaccines without refrigeration.NPJ vaccines · 2023Article
- Inhibition of Cytomegalovirus byViruses · 2023Article
- Anhydrous Nucleic Acid Nanoparticles for Storage and Handling at Broad Range of Temperatures.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Ultraviolet dosage and decontamination efficacy were widely variable across 14 UV devices after testing a dried enveloped ribonucleic acid virus surrogate for SARS-CoV-2.Frontiers in bioengineering and biotechnology · 2022Article
- Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation.Journal of virological methods · 2021Article
- Stability of Chimpanzee Adenovirus Vectored Vaccines (ChAdOx1 and ChAdOx2) in Liquid and Lyophilised Formulations.Vaccines · 2021Article
- Manufacturing Bacteriophages (Part 2 of 2): Formulation, Analytics and Quality Control Considerations.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus.Journal of visualized experiments : JoVE · 2021Article
- Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens.Vaccines · 2021Article
- Prospects of Inhaled Phage Therapy for Combatting Pulmonary Infections.Frontiers in cellular and infection microbiology · 2021Review
- Coronavirus Persistence on a Plastic Carrier Under Refrigeration Conditions and Its Reduction Using Wet Wiping Technique, with Respect to Food Safety.Food and environmental virology · 2020Article
- Improved lyophilization conditions for long-term storage of bacteriophages.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo make a comparative evaluation of the effects of different stabilizers and freezing rates on structurally different viruses during lyophilization and storage. METHODS AND
resultsTwo virus strains from each of six animal virus families, including both enveloped and nonenveloped viruses, were lyophilized in (i) culture medium, (ii) with the addition of gelatine-sucrose and (iii) skim milk-sodium glutamate. All the virus suspensions were frozen (i) at -80°C or (ii) in liquid nitrogen before lyophilization. Virus titre assay after lyophilization and after 8 months storage at 4°C revealed that the efficacy of stabilizers depended on virus structure. Generally, the best protective quality for enveloped viruses was achieved with gelatine-sucrose, which best maintained their infectivity and envelope morphology. Even additive-free culture medium proved adequate for nonenveloped viruses. Differences in stabilizer efficacy were also found between virus families and were expressed immediately after lyophilization; the activity of stabilizers in the course of storage was very similar. Freezing in liquid nitrogen proved beneficial for picornaviruses.
conclusionsThe choice of an appropriate stabilizer with respect to virus type is crucial for effective lyophilization. SIGNIFICANCE AND IMPACT OF THE STUDY: This study contributes to the establishment of general guidelines for animal virus lyophilization, with particular respect to differences in virus structure.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.