Evidence map›Paper›PMID 25249934›Full record

ArticleFrontiers in cellular neuroscience2014

Impurity of stem cell graft by murine embryonic fibroblasts - implications for cell-based therapy of the central nervous system.

Marek Molcanyi, Narges Zare Mehrjardi, Ute Schäfer, Nadia Nabil Haj-Yasein, Michael Brockmann, Marina Penner, Peter Riess, Clemens Reinshagen, Bernhard Rieger, Tobias Hannes and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Mechanism of Endoplasmic Reticulum Stress in Cerebral Ischemia.Frontiers in cellular neuroscience · 2021
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Marek MolcanyiInstitute of Neurophysiology, Medical Faculty, University of Cologne , Cologne , Germany ; Clinic of Neurosurgery, Medical Faculty, University of Cologne , Cologne , Germany.
Narges Zare MehrjardiInstitute of Neurophysiology, Medical Faculty, University of Cologne , Cologne , Germany.
Ute SchäferResearch Unit for Experimental Neurotraumatology, Medical University of Graz , Graz , Austria.
Nadia Nabil Haj-YaseinDepartment of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo , Oslo , Norway.
Michael BrockmannDepartment of Pathology, Kliniken der Stadt Köln, Cologne-Merheim Hospital, University of Witten/Herdecke , Cologne , Germany.
Marina PennerClinic of Neurosurgery, Medical Faculty, University of Cologne , Cologne , Germany.
Peter RiessDepartment of Traumatology and Orthopedics, HELIOS Klinik Bad Berleburg , Bad Berleburg , Germany.
Clemens ReinshagenMolecular Neurotherapy and Imaging Laboratory, Massachusetts General Hospital, Harvard Medical School , Boston, MA , USA ; Department of Radiology, Massachusetts General Hospital, Harvard Medical School , Boston, MA , USA.
Bernhard RiegerClinic of Neurosurgery, Medical Faculty, University of Cologne , Cologne , Germany.
Tobias HannesInstitute of Neurophysiology, Medical Faculty, University of Cologne , Cologne , Germany ; Department of Pediatric Cardiology, Heart Center Cologne, Medical Faculty, University Hospital of Cologne , Cologne , Germany.
Jürgen HeschelerInstitute of Neurophysiology, Medical Faculty, University of Cologne , Cologne , Germany.
Bert BoscheDivision of Neurosurgery, St Michael's Hospital, Keenan Research Centre for Biomedical Science and the Li Ka Shing Knowledge Institute of St. Michael's Hospital, Department of Surgery, University of Toronto , Toronto, ON , Canada ; Department of Neurology, University Hospital of Essen, University of Duisburg-Essen , Essen , Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. The types of implanted cells appear to play a crucial role. Previously, groups of the stem cell network NRW implemented a feeder-based cell line within the scope of their projects, examining the implantation of stem cells after ischemic stroke and traumatic brain injury. Retrospective evaluation indicated the presence of spindle-shaped cells in several grafts implanted in injured animals, which indicated potential contamination by co-cultured feeder cells (murine embryonic fibroblasts - MEFs). Because feeder-based cell lines have been previously exposed to a justified criticism with regard to contamination by animal glycans, we aimed to evaluate the effects of stem cell/MEF co-transplantation. MEFs accounted for 5.3 ± 2.8% of all cells in the primary FACS-evaluated co-culture. Depending on the culture conditions and subsequent purification procedure, the MEF-fraction ranged from 0.9 to 9.9% of the cell suspensions in vitro. MEF survival and related formation of extracellular substances in vivo were observed after implantation into the uninjured rat brain. Impurity of the stem cell graft by MEFs interferes with translational strategies, which represents a threat to the potential recipient and may affect the graft microenvironment. The implications of these findings are critically discussed.

Indexed as

brain injurycell graft contaminationfeeder-based cell linemurine embryonic fibroblastsstem cell transplantationstroke

Identifiers

PMID25249934
PMCPMC4155790

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.