ArticleGenetics and molecular biology2014
Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp.
Article in Genetics and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Primary Culture and Characterization of a Crucian Carp (Animals : an open access journal from MDPI · 2026Article
- Transcriptome Profile Analyses of Head Kidney in Roach (Biology · 2023Article
- Innate Immune Responses of Skin Mucosa in Common Carp (Animals : an open access journal from MDPI · 2020Article
- Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.Molecular biology reports · 2018Article
- Identification of reference genes for qRT-PCR in granulosa cells of healthy women and polycystic ovarian syndrome patients.Scientific reports · 2017Article
- Simvastatin effects on detoxification mechanisms in Danio rerio embryos.Environmental science and pollution research international · 2016Article
- Weaning Markedly Affects Transcriptome Profiles and Peyer's Patch Development in Piglet Ileum.Frontiers in immunology · 2015Article
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Authors and funding
5 authors.
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Abstract
Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across different tissue types or under different experimental conditions. To identify the most suitable reference genes for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus) under various conditions (sex, age, water temperature, and drug treatments), seven reference genes, including beta actin (ACTB), beta-2 microglobulin (B2M), embryonic elongation factor-1 alpha (EEF1A), glyceraldehyde phosphate dehydrogenase (GAPDH), alpha tubulin (TUBA), ribosomal protein l8 (RPL8) and glucose-6-phosphate dehydrogenase (G6PDH), were evaluated in this study. The stability and ranking of gene expression were analyzed using three different statistical programs: GeNorm, Normfinder and Bestkeeper. The expression errors associated with selection of the genes were assessed by the relative quantity of CYP4T. The results indicated that all the seven genes exhibited variability under the experimental conditions of this research, and the combination of ACTB/TUBA/EEF1A or of ACTB/EEF1A was the best candidate that raised the accuracy of quantitative analysis of gene expression. The findings highlighted the importance of validation of housekeeping genes for research on gene expression under different conditions of experiment and species.
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